Assay Detail
Binding
CHEMBL5716073
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Itk in human Jurkat T cells assessed as inhibition of IL-2 secretion incubated for 16 hrs in presence of anti-CD3 antibody G19-4 by ELISA analysis
4
Total Activities
2
Compounds Tested
2
Activity Types
4
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Jurkat T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Selective Itk inhibitors block T-cell activation and murine lung inflammation.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 2 | - | - |
| IC50 | 2 | 6.38 | 6.60 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | EFO:0005140 |
| EFO_ID | EFO_ID | - | — | HP:0002099 |
| EFO_TERM | EFO_TERM | - | — | autoimmune disease |
| EFO_TERM | EFO_TERM | - | — | asthma |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL209148 | — | — | 2 | 6.60 |
| CHEMBL382362 | — | — | 2 | 6.16 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL209148 | — | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL382362 | — | IC50 | = | 700.0 | nM | 6.16 |
| CHEMBL382362 | — | Inhibition | - | % | - | |
| CHEMBL209148 | — | Inhibition | - | % | - |