Assay Detail
Binding
CHEMBL5717028
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to BTZO-12 sensor chip immobilized human MIF expressed in Escherichia coli BL21 (DE3) by SPR analysis
1
Total Activities
1
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 1 | 7.06 | 7.06 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | EFO:0003777 |
| EFO_TERM | EFO_TERM | - | — | heart disease |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4516611 | BTZO-1 | — | 1 | 7.06 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4516611 | BTZO-1 | Kd | = | 88.0 | nM | 7.06 |