Assay Detail
Binding
CHEMBL5717040
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of [3H]-BTZO-1 binding to recombinant human C-terminal His-tagged MIF expressed in Escherichia coli BL21 (DE3) measured after 3 hrs by SPA
5
Total Activities
5
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 5.79 | 7.15 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | EFO:0003777 |
| EFO_TERM | EFO_TERM | - | — | heart disease |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4516611 | BTZO-1 | — | 1 | 7.15 |
| CHEMBL242080 | E319 | — | 1 | 5.96 |
| CHEMBL482477 | — | — | 1 | 5.44 |
| CHEMBL1627201 | L-SULFORAPHANE | 2.0 | 1 | 4.60 |
| CHEMBL210858 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4516611 | BTZO-1 | IC50 | = | 71.0 | nM | 7.15 |
| CHEMBL242080 | E319 | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL482477 | — | IC50 | = | 3600.0 | nM | 5.44 |
| CHEMBL1627201 | L-SULFORAPHANE | IC50 | = | 25000.0 | nM | 4.60 |
| CHEMBL210858 | — | IC50 | > | 100000.0 | nM | - |