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Assay Detail

CHEMBL5729906

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Kinase Screen Assay: KINOMEscan (Ambit Biosciences, San Diego, Calif.), a high-throughput method for screening small molecular agents against a large panel of human kinases, was utilized for compound 2. The technology is a competition binding assay that profiled the selectivity of compound 2 against 350 kinases, each fused to a proprietary tag. The quantity of each kinase bound to an immobilized, active site-directed ligand was measured in the presence and absence of compound 2.
3
Total Activities
1
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fused 2-aminothiazole compounds
(2014)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 1 8.64 8.64
kon 1 - -
k_off 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2148124 3 8.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2148124 Kd = 2.3 nM 8.64
CHEMBL2148124 kon = - -
CHEMBL2148124 k_off = - s-1 -