Assay Detail
Binding
CHEMBL5729932
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzyme Assay: Compounds for testing were diluted in DMSO to 50 fold the final concentration and a ten point three fold dilution series was made. The compounds were diluted in assay buffer (50 mM HEPES, pH 7.4, 100 mM KCl, 0.001% Tween-20, 0.05% BSA, 20 uM TCEP) to 6 fold their final concentration. The HDAC enzymes (purchased from BPS Biosciences) were diluted to 1.5 fold their final concentration in assay buffer. The tripeptide substrate and trypsin at 0.05 uM final concentration were diluted in assay buffer at 6 fold their final concentration. The final enzyme concentrations used in these assays were 3.3 ng/ml (HDAC1), 0.2 ng/ml (HDAC2), 0.08 ng/ml (HDAC3) and 2 ng/ml (HDAC6). The final substrate concentrations used were 16 uM (HDAC1), 10 uM (HDAC2), 17 uM (HDAC3) and 14 uM (HDAC6). Five ul of compounds and 20 ul of enzyme were added to wells of a black, opaque 384 well plate in duplicate. Enzyme and compound were incubated together at room temperature.
423
Total Activities
138
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Pyrimidine hydroxy amide compounds as protein deacetylase inhibitors and methods of use thereof
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 141 | 8.30 | 9.00 |
| kon | 141 | - | - |
| k_off | 141 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3655944 | — | — | 6 | 9.00 |
| CHEMBL3655967 | — | — | 3 | 9.00 |
| CHEMBL3655993 | — | — | 3 | 9.00 |
| CHEMBL3655917 | — | — | 3 | 9.00 |
| CHEMBL3655957 | — | — | 3 | 8.70 |
| CHEMBL3655997 | — | — | 3 | 8.70 |
| CHEMBL3655996 | — | — | 3 | 8.70 |
| CHEMBL3655995 | — | — | 3 | 8.70 |
| CHEMBL3655994 | — | — | 3 | 8.70 |
| CHEMBL3655990 | — | — | 3 | 8.70 |
| CHEMBL3655984 | — | — | 3 | 8.70 |
| CHEMBL3655979 | — | — | 3 | 8.70 |
| CHEMBL3655974 | — | — | 3 | 8.70 |
| CHEMBL3655971 | — | — | 3 | 8.70 |
| CHEMBL3655968 | — | — | 3 | 8.70 |
| CHEMBL3655964 | — | — | 3 | 8.70 |
| CHEMBL3655959 | — | — | 3 | 8.70 |
| CHEMBL3655950 | — | — | 3 | 8.70 |
| CHEMBL3655948 | — | — | 3 | 8.70 |
| CHEMBL3655942 | — | — | 3 | 8.70 |
| CHEMBL3655936 | — | — | 3 | 8.70 |
| CHEMBL3655932 | — | — | 3 | 8.70 |
| CHEMBL3655929 | — | — | 3 | 8.70 |
| CHEMBL3655923 | — | — | 3 | 8.70 |
| CHEMBL3655919 | — | — | 3 | 8.70 |
| CHEMBL3655918 | — | — | 3 | 8.70 |
| CHEMBL3655916 | — | — | 3 | 8.70 |
| CHEMBL3655915 | — | — | 6 | 8.70 |
| CHEMBL3652242 | — | — | 3 | 8.52 |
| CHEMBL3652243 | — | — | 3 | 8.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3655944 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3655917 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3655993 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3655967 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3655929 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655950 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655997 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655995 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655948 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655944 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655996 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655968 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655971 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655994 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655915 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655942 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655964 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655923 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655979 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655932 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655957 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655915 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655959 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655916 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655918 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655974 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655919 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655984 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655936 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655990 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3655998 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655952 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655935 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3656017 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655966 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655999 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3656000 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3656015 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655970 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3652242 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3656003 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3656013 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3652243 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655960 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3656005 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655947 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655913 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655992 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3655958 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL3656020 | — | IC50 | = | 3.0 | nM | 8.52 |