Assay Detail
Binding
CHEMBL5729989
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Kinase Assay: Kinase assays were performed using the assay kit by Reaction Biology Corp (Malvern, Pa.). The compounds were tested at 10 concentrations by 3-fold serial dilutions starting at 30 μM. Staurosporin was used as a control nonspecific kinase inhibitor. In vitro kinase reactions using purified kinases were carried out in the presence of 10 μM ATP and test compounds.
12
Total Activities
4
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Compounds and methods useful for directing stem cell differentiation
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 5.76 | 6.25 |
| kon | 4 | - | - |
| k_off | 4 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2385590 | — | — | 3 | 6.25 |
| CHEMBL3668019 | — | — | 3 | 6.00 |
| CHEMBL3668018 | — | — | 3 | 5.80 |
| CHEMBL478629 | DORSOMORPHIN | — | 3 | 4.97 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2385590 | — | IC50 | = | 565.0 | nM | 6.25 |
| CHEMBL3668019 | — | IC50 | = | 998.99 | nM | 6.00 |
| CHEMBL3668018 | — | IC50 | = | 1578.0 | nM | 5.80 |
| CHEMBL478629 | DORSOMORPHIN | IC50 | = | 10760.0 | nM | 4.97 |
| CHEMBL3668018 | — | kon | = | - | — | - |
| CHEMBL3668018 | — | k_off | = | - | s-1 | - |
| CHEMBL3668019 | — | kon | = | - | — | - |
| CHEMBL3668019 | — | k_off | = | - | s-1 | - |
| CHEMBL2385590 | — | kon | = | - | — | - |
| CHEMBL2385590 | — | k_off | = | - | s-1 | - |
| CHEMBL478629 | DORSOMORPHIN | kon | = | - | — | - |
| CHEMBL478629 | DORSOMORPHIN | k_off | = | - | s-1 | - |