Assay Detail
Binding
CHEMBL5730140
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzyme Assay: The assay has been carried out in 96 well format and the BIOMOL fluorescent-based HDAC activity assay has been applied. The reaction composed of assay buffer, containing 25 mM Tris pH 7.5, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/ml BSA, tested compounds, 500 nM HDAC8 enzyme or 600 nM HDAC1 enzyme, 200 μM Flur de lys p53 peptide substrate for HDAC8 enzyme or 500 μM Flur de lys generic substrate for HDAC1 enzyme and subsequently was incubated at room temperature for 2 h. Flur de lys Developer was added and the reaction was incubated for 10 min. Briefly, deacetylation of the substrate sensitizes it to the developer, which then generates a fluorophore (symbol). The fluorophore is excited with 360 nm light and the emitted light (460 nm) is detected on a fluorometric plate reader.
288
Total Activities
96
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Benzimidazole derivatives: preparation and pharmaceutical applications
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 96 | 6.46 | 7.62 |
| kon | 96 | - | - |
| k_off | 96 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL460963 | — | — | 3 | 7.62 |
| CHEMBL491688 | — | — | 3 | 7.58 |
| CHEMBL3648287 | — | — | 3 | 7.52 |
| CHEMBL489332 | — | — | 3 | 7.41 |
| CHEMBL474746 | — | — | 3 | 7.29 |
| CHEMBL3648290 | — | — | 3 | 7.28 |
| CHEMBL491316 | — | — | 3 | 7.28 |
| CHEMBL3648280 | — | — | 3 | 7.27 |
| CHEMBL3648291 | — | — | 3 | 7.21 |
| CHEMBL3648305 | — | — | 3 | 7.21 |
| CHEMBL3648289 | — | — | 3 | 7.19 |
| CHEMBL475145 | — | — | 3 | 7.19 |
| CHEMBL3648279 | — | — | 3 | 7.15 |
| CHEMBL491491 | — | — | 3 | 7.14 |
| CHEMBL3648267 | — | — | 3 | 7.04 |
| CHEMBL5811849 | — | — | 3 | 7.00 |
| CHEMBL3648306 | — | — | 3 | 6.94 |
| CHEMBL3648285 | — | — | 3 | 6.94 |
| CHEMBL3648292 | — | — | 3 | 6.90 |
| CHEMBL3645375 | — | — | 3 | 6.88 |
| CHEMBL3648281 | — | — | 3 | 6.88 |
| CHEMBL5907207 | — | — | 3 | 6.86 |
| CHEMBL3648308 | — | — | 3 | 6.84 |
| CHEMBL474349 | — | — | 3 | 6.82 |
| CHEMBL523601 | — | — | 3 | 6.80 |
| CHEMBL5906708 | — | — | 3 | 6.80 |
| CHEMBL489331 | — | — | 3 | 6.79 |
| CHEMBL3648286 | — | — | 3 | 6.78 |
| CHEMBL3648288 | — | — | 3 | 6.78 |
| CHEMBL442585 | — | — | 3 | 6.78 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL460963 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL491688 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL3648287 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL489332 | — | IC50 | = | 39.0 | nM | 7.41 |
| CHEMBL474746 | — | IC50 | = | 51.0 | nM | 7.29 |
| CHEMBL3648290 | — | IC50 | = | 52.0 | nM | 7.28 |
| CHEMBL491316 | — | IC50 | = | 52.0 | nM | 7.28 |
| CHEMBL3648280 | — | IC50 | = | 54.0 | nM | 7.27 |
| CHEMBL3648305 | — | IC50 | = | 62.0 | nM | 7.21 |
| CHEMBL3648291 | — | IC50 | = | 61.0 | nM | 7.21 |
| CHEMBL475145 | — | IC50 | = | 64.0 | nM | 7.19 |
| CHEMBL3648289 | — | IC50 | = | 65.0 | nM | 7.19 |
| CHEMBL3648279 | — | IC50 | = | 71.0 | nM | 7.15 |
| CHEMBL491491 | — | IC50 | = | 72.0 | nM | 7.14 |
| CHEMBL3648267 | — | IC50 | = | 92.0 | nM | 7.04 |
| CHEMBL5811849 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL3648285 | — | IC50 | = | 116.0 | nM | 6.94 |
| CHEMBL3648306 | — | IC50 | = | 116.0 | nM | 6.94 |
| CHEMBL3648292 | — | IC50 | = | 125.0 | nM | 6.90 |
| CHEMBL3645375 | — | IC50 | = | 131.0 | nM | 6.88 |
| CHEMBL3648281 | — | IC50 | = | 131.0 | nM | 6.88 |
| CHEMBL5907207 | — | IC50 | = | 137.0 | nM | 6.86 |
| CHEMBL3648308 | — | IC50 | = | 146.0 | nM | 6.84 |
| CHEMBL474349 | — | IC50 | = | 153.0 | nM | 6.82 |
| CHEMBL5906708 | — | IC50 | = | 160.0 | nM | 6.80 |
| CHEMBL523601 | — | IC50 | = | 158.0 | nM | 6.80 |
| CHEMBL489331 | — | IC50 | = | 162.0 | nM | 6.79 |
| CHEMBL3648312 | — | IC50 | = | 167.0 | nM | 6.78 |
| CHEMBL464719 | — | IC50 | = | 167.0 | nM | 6.78 |
| CHEMBL442585 | — | IC50 | = | 165.0 | nM | 6.78 |
| CHEMBL3648288 | — | IC50 | = | 168.0 | nM | 6.78 |
| CHEMBL3648286 | — | IC50 | = | 166.0 | nM | 6.78 |
| CHEMBL474152 | — | IC50 | = | 171.0 | nM | 6.77 |
| CHEMBL515906 | — | IC50 | = | 173.0 | nM | 6.76 |
| CHEMBL489706 | — | IC50 | = | 194.0 | nM | 6.71 |
| CHEMBL3648316 | — | IC50 | = | 196.0 | nM | 6.71 |
| CHEMBL523646 | — | IC50 | = | 195.0 | nM | 6.71 |
| CHEMBL1377382 | — | IC50 | = | 195.0 | nM | 6.71 |
| CHEMBL3648268 | — | IC50 | = | 206.0 | nM | 6.69 |
| CHEMBL521909 | — | IC50 | = | 206.0 | nM | 6.69 |
| CHEMBL490721 | — | IC50 | = | 218.0 | nM | 6.66 |
| CHEMBL489125 | — | IC50 | = | 227.0 | nM | 6.64 |
| CHEMBL6030983 | — | IC50 | = | 230.0 | nM | 6.64 |
| CHEMBL3648318 | — | IC50 | = | 234.0 | nM | 6.63 |
| CHEMBL491490 | — | IC50 | = | 246.0 | nM | 6.61 |
| CHEMBL489709 | — | IC50 | = | 268.0 | nM | 6.57 |
| CHEMBL5907529 | — | IC50 | = | 290.0 | nM | 6.54 |
| CHEMBL3645371 | — | IC50 | = | 286.0 | nM | 6.54 |
| CHEMBL3648294 | — | IC50 | = | 294.0 | nM | 6.53 |
| CHEMBL3648284 | — | IC50 | = | 297.0 | nM | 6.53 |