Assay Detail
Binding
CHEMBL5730189
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In Vitro HotSpot Kinase Assay: IC50s were determined using the in vitro HotSpot kinase assay (purified enzymes, 33P-ATP, an appropriate substrate and 1 uM ATP.). Reaction conditions were 1 uM ATP, one hour incubation with inhibitor, and kinase activity detected using 33-ATP phosphorylation of an appropriately selected peptide substrate.
12
Total Activities
2
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Receptor tyrosine-protein kinase erbB-4 (CHEMBL3009) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibitors of bruton's tyrosine kinase for the treatment of solid tumors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 8.08 | 10.00 |
| kon | 4 | - | - |
| k_off | 4 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1873475 | IBRUTINIB | 4.0 | 6 | 10.00 |
| CHEMBL3647967 | — | — | 6 | 8.49 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1873475 | IBRUTINIB | IC50 | = | 0.1 | nM | 10.00 |
| CHEMBL3647967 | — | IC50 | = | 3.2 | nM | 8.49 |
| CHEMBL1873475 | IBRUTINIB | IC50 | = | 9.4 | nM | 8.03 |
| CHEMBL3647967 | — | IC50 | = | 1536.0 | nM | 5.81 |
| CHEMBL1873475 | IBRUTINIB | kon | = | - | — | - |
| CHEMBL1873475 | IBRUTINIB | k_off | = | - | s-1 | - |
| CHEMBL1873475 | IBRUTINIB | kon | = | - | — | - |
| CHEMBL1873475 | IBRUTINIB | k_off | = | - | s-1 | - |
| CHEMBL3647967 | — | kon | = | - | — | - |
| CHEMBL3647967 | — | k_off | = | - | s-1 | - |
| CHEMBL3647967 | — | kon | = | - | — | - |
| CHEMBL3647967 | — | k_off | = | - | s-1 | - |