Assay Detail
Binding
CHEMBL5731333
Review assay metadata, readout intent, target linkage, and publication context from the same page.
P70S6K Enzyme Assay: P70S6K inhibitor compounds were diluted and plated in 96 well plates. A reaction mixture including the following components was then added to the compound plate to initiate the enzyme reaction; P70S6K (3 nM, T412E mutant, Millipore) was mixed with 24 μM ATP in an assay buffer containing 100 mM Hepes (pH 7.5), 5 mM MgCl2, 1 mM DTT, 0.015% Brij and 1 μM of the substrate peptide FITC-AHA-AKRRRLSSLRA-OH (derived from the S6 ribosomal protein sequence, FITC=fluorescein isothiocyanate, AHA=6-aminohexanoic acid). The reaction was incubated for 90 min at 25° C., before the addition of 10 mM EDTA to stop the reaction. The proportion of substrate and product (phosphorylated) peptide was analysed on a Caliper Life Sciences Lab Chip 3000, using a pressure of −1.4 psi, and upstream and downstream voltages of −3000 and −700 respectively. Product peaks were resolved before substrate peaks on the resulting chromatograms.
729
Total Activities
243
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Aminopyrimidine derivatives for use as modulators of kinase activity
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 243 | 6.93 | 9.10 |
| kon | 243 | - | - |
| k_off | 243 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4106847 | — | — | 3 | 9.10 |
| CHEMBL3929756 | — | — | 3 | 9.05 |
| CHEMBL3942270 | — | — | 3 | 8.89 |
| CHEMBL3896291 | — | — | 3 | 8.85 |
| CHEMBL3896704 | — | — | 3 | 8.77 |
| CHEMBL3957511 | — | — | 3 | 8.70 |
| CHEMBL3982464 | — | — | 3 | 8.66 |
| CHEMBL3964354 | — | — | 3 | 8.60 |
| CHEMBL4110986 | — | — | 3 | 8.59 |
| CHEMBL3964273 | — | — | 3 | 8.59 |
| CHEMBL3934983 | — | — | 3 | 8.59 |
| CHEMBL3936890 | — | — | 3 | 8.55 |
| CHEMBL4114630 | — | — | 3 | 8.49 |
| CHEMBL3911701 | — | — | 3 | 8.48 |
| CHEMBL3917646 | — | — | 3 | 8.48 |
| CHEMBL4110173 | — | — | 3 | 8.47 |
| CHEMBL3906331 | — | — | 3 | 8.47 |
| CHEMBL3932608 | — | — | 3 | 8.47 |
| CHEMBL3915352 | — | — | 3 | 8.40 |
| CHEMBL3972358 | — | — | 3 | 8.39 |
| CHEMBL3948645 | — | — | 3 | 8.38 |
| CHEMBL3920854 | — | — | 3 | 8.36 |
| CHEMBL3903649 | — | — | 3 | 8.35 |
| CHEMBL3935973 | — | — | 3 | 8.35 |
| CHEMBL3930957 | — | — | 3 | 8.34 |
| CHEMBL3905652 | — | — | 3 | 8.33 |
| CHEMBL3929477 | — | — | 3 | 8.32 |
| CHEMBL3928625 | — | — | 3 | 8.31 |
| CHEMBL4111671 | — | — | 3 | 8.31 |
| CHEMBL3947951 | — | — | 3 | 8.28 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4106847 | — | IC50 | = | 0.8 | nM | 9.10 |
| CHEMBL3929756 | — | IC50 | = | 0.9 | nM | 9.05 |
| CHEMBL3942270 | — | IC50 | = | 1.3 | nM | 8.89 |
| CHEMBL3896291 | — | IC50 | = | 1.4 | nM | 8.85 |
| CHEMBL3896704 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL3957511 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3982464 | — | IC50 | = | 2.2 | nM | 8.66 |
| CHEMBL3964354 | — | IC50 | = | 2.5 | nM | 8.60 |
| CHEMBL4110986 | — | IC50 | = | 2.6 | nM | 8.59 |
| CHEMBL3964273 | — | IC50 | = | 2.6 | nM | 8.59 |
| CHEMBL3934983 | — | IC50 | = | 2.6 | nM | 8.59 |
| CHEMBL3936890 | — | IC50 | = | 2.8 | nM | 8.55 |
| CHEMBL4114630 | — | IC50 | = | 3.2 | nM | 8.49 |
| CHEMBL3911701 | — | IC50 | = | 3.3 | nM | 8.48 |
| CHEMBL3917646 | — | IC50 | = | 3.3 | nM | 8.48 |
| CHEMBL3932608 | — | IC50 | = | 3.4 | nM | 8.47 |
| CHEMBL3906331 | — | IC50 | = | 3.4 | nM | 8.47 |
| CHEMBL4110173 | — | IC50 | = | 3.4 | nM | 8.47 |
| CHEMBL3915352 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3972358 | — | IC50 | = | 4.1 | nM | 8.39 |
| CHEMBL3948645 | — | IC50 | = | 4.2 | nM | 8.38 |
| CHEMBL3920854 | — | IC50 | = | 4.4 | nM | 8.36 |
| CHEMBL3903649 | — | IC50 | = | 4.5 | nM | 8.35 |
| CHEMBL3935973 | — | IC50 | = | 4.5 | nM | 8.35 |
| CHEMBL3930957 | — | IC50 | = | 4.6 | nM | 8.34 |
| CHEMBL3905652 | — | IC50 | = | 4.7 | nM | 8.33 |
| CHEMBL3929477 | — | IC50 | = | 4.8 | nM | 8.32 |
| CHEMBL4111671 | — | IC50 | = | 4.9 | nM | 8.31 |
| CHEMBL3928625 | — | IC50 | = | 4.9 | nM | 8.31 |
| CHEMBL3947951 | — | IC50 | = | 5.3 | nM | 8.28 |
| CHEMBL3913380 | — | IC50 | = | 5.3 | nM | 8.28 |
| CHEMBL3933677 | — | IC50 | = | 5.2 | nM | 8.28 |
| CHEMBL3918502 | — | IC50 | = | 5.8 | nM | 8.24 |
| CHEMBL3957113 | — | IC50 | = | 5.8 | nM | 8.24 |
| CHEMBL3892901 | — | IC50 | = | 5.7 | nM | 8.24 |
| CHEMBL3906780 | — | IC50 | = | 5.9 | nM | 8.23 |
| CHEMBL3928221 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3929706 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL3955537 | — | IC50 | = | 6.1 | nM | 8.21 |
| CHEMBL3890900 | — | IC50 | = | 6.3 | nM | 8.20 |
| CHEMBL3916166 | — | IC50 | = | 7.2 | nM | 8.14 |
| CHEMBL3933781 | — | IC50 | = | 8.5 | nM | 8.07 |
| CHEMBL3897935 | — | IC50 | = | 9.1 | nM | 8.04 |
| CHEMBL3963726 | — | IC50 | = | 9.5 | nM | 8.02 |
| CHEMBL3942101 | — | IC50 | = | 9.8 | nM | 8.01 |
| CHEMBL3978726 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3986206 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3940443 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3909882 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL3980046 | — | IC50 | = | 12.0 | nM | 7.92 |