Assay Detail
Binding
CHEMBL5731361
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Surface Plasmon Resonance (SPR) Assay: Binding of inhibitors to expressed cyclophilins was determined using surface plasmon resonance (SPR) experiments. Briefly, avi-tagged cyclophilin proteins with mono-biotinylation were immobilized onto a Biotin CAPture chip (GE Healthcare, cat. #28920234). SPR experiments were carried out on an upgraded Biacore T200 system using a running buffer containing the inhibitor, 10 mM HEPES, pH 7.4, 150 mM NaCl, 0.05% P20, and 3% DMSO. Single-cycle kinetics measurements were used to study the interactions between cyclophilin inhibitors and different cyclophlins. Accordingly, compound at various concentrations was injected to the flow cells with a contact time of 1 min each at a flow rate of 50 μL/min. The final dissociation phase lasts for 10 min following the sample injection. Data was analyzed using Biacore T200 Evaluation Software Version 1.0 and a 1:1 binding model was applied to fit the data to obtain kon, koff, and KD.
321
Total Activities
105
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peptidyl-prolyl cis-trans isomerase A (CHEMBL1949) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Cyclic peptides and use as medicines
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 107 | 8.81 | 9.30 |
| kon | 107 | - | - |
| k_off | 107 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5792144 | — | — | 3 | 9.30 |
| CHEMBL6051666 | — | — | 3 | 9.30 |
| CHEMBL5759368 | — | — | 3 | 9.30 |
| CHEMBL5812164 | — | — | 3 | 9.30 |
| CHEMBL5791301 | — | — | 3 | 9.22 |
| CHEMBL5994784 | — | — | 3 | 9.22 |
| CHEMBL6062788 | — | — | 3 | 9.22 |
| CHEMBL5810211 | — | — | 3 | 9.22 |
| CHEMBL5942857 | — | — | 3 | 9.22 |
| CHEMBL3344501 | — | — | 3 | 9.22 |
| CHEMBL6010886 | — | — | 3 | 9.22 |
| CHEMBL6059588 | — | — | 3 | 9.22 |
| CHEMBL5856868 | — | — | 3 | 9.22 |
| CHEMBL5757514 | — | — | 3 | 9.15 |
| CHEMBL6026994 | — | — | 3 | 9.15 |
| CHEMBL5866658 | — | — | 3 | 9.15 |
| CHEMBL6003202 | — | — | 3 | 9.15 |
| CHEMBL6056488 | — | — | 3 | 9.15 |
| CHEMBL5831563 | — | — | 3 | 9.10 |
| CHEMBL5842043 | — | — | 3 | 9.10 |
| CHEMBL6048373 | — | — | 3 | 9.10 |
| CHEMBL5741417 | — | — | 3 | 9.10 |
| CHEMBL5985508 | — | — | 3 | 9.05 |
| CHEMBL5967576 | — | — | 3 | 9.05 |
| CHEMBL5992700 | — | — | 3 | 9.05 |
| CHEMBL6038296 | — | — | 3 | 9.05 |
| CHEMBL5976250 | — | — | 3 | 9.05 |
| CHEMBL5867542 | — | — | 3 | 9.05 |
| CHEMBL6023421 | — | — | 3 | 9.05 |
| CHEMBL5812196 | — | — | 3 | 9.05 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5812164 | — | Kd | = | 0.5 | nM | 9.30 |
| CHEMBL5792144 | — | Kd | = | 0.5 | nM | 9.30 |
| CHEMBL5759368 | — | Kd | = | 0.5 | nM | 9.30 |
| CHEMBL6051666 | — | Kd | = | 0.5 | nM | 9.30 |
| CHEMBL5810211 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL5942857 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL3344501 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL6059588 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL6062788 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL5994784 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL6010886 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL5791301 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL5856868 | — | Kd | = | 0.6 | nM | 9.22 |
| CHEMBL5866658 | — | Kd | = | 0.7 | nM | 9.15 |
| CHEMBL6056488 | — | Kd | = | 0.7 | nM | 9.15 |
| CHEMBL6026994 | — | Kd | = | 0.7 | nM | 9.15 |
| CHEMBL6003202 | — | Kd | = | 0.7 | nM | 9.15 |
| CHEMBL5757514 | — | Kd | = | 0.7 | nM | 9.15 |
| CHEMBL6048373 | — | Kd | = | 0.8 | nM | 9.10 |
| CHEMBL5842043 | — | Kd | = | 0.8 | nM | 9.10 |
| CHEMBL5741417 | — | Kd | = | 0.8 | nM | 9.10 |
| CHEMBL5831563 | — | Kd | = | 0.8 | nM | 9.10 |
| CHEMBL6023421 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL5812196 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL5867542 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL6044098 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL5985508 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL5967576 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL5976250 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL5992700 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL6038296 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL6058387 | — | Kd | = | 0.9 | nM | 9.05 |
| CHEMBL5798116 | — | Kd | = | 1.0 | nM | 9.00 |
| CHEMBL6062808 | — | Kd | = | 1.0 | nM | 9.00 |
| CHEMBL5974662 | — | Kd | = | 1.0 | nM | 9.00 |
| CHEMBL5821248 | — | Kd | = | 1.0 | nM | 9.00 |
| CHEMBL6023618 | — | Kd | = | 1.1 | nM | 8.96 |
| CHEMBL5960352 | — | Kd | = | 1.1 | nM | 8.96 |
| CHEMBL5859080 | — | Kd | = | 1.1 | nM | 8.96 |
| CHEMBL6018548 | — | Kd | = | 1.1 | nM | 8.96 |
| CHEMBL5744117 | — | Kd | = | 1.2 | nM | 8.92 |
| CHEMBL3344493 | — | Kd | = | 1.2 | nM | 8.92 |
| CHEMBL5926312 | — | Kd | = | 1.2 | nM | 8.92 |
| CHEMBL5960352 | — | Kd | = | 1.2 | nM | 8.92 |
| CHEMBL6037963 | — | Kd | = | 1.3 | nM | 8.89 |
| CHEMBL3344499 | — | Kd | = | 1.3 | nM | 8.89 |
| CHEMBL5877920 | — | Kd | = | 1.3 | nM | 8.89 |
| CHEMBL5820903 | — | Kd | = | 1.3 | nM | 8.89 |
| CHEMBL5904243 | — | Kd | = | 1.3 | nM | 8.89 |
| CHEMBL5887631 | — | Kd | = | 1.3 | nM | 8.89 |