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Assay Detail

CHEMBL5731393

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
E-VIPR Optical Membrane Potential Assay: Sodium channels are voltage-dependent proteins that can be activated by inducing membrane voltage changes by applying electric fields. The electrical stimulation instrument and methods of use are described in Ion Channel Assay Methods PCT/US01/21652, herein incorporated by reference and are referred to as E-VIPR. The instrument comprises a microtiter plate handler, an optical system for exciting the coumarin dye while simultaneously recording the coumarin and oxonol emissions, a waveform generator, a current- or voltage-controlled amplifier, and a device for inserting electrodes in well. Under integrated computer control, this instrument passes user-programmed electrical stimulus protocols to cells within the wells of the microtiter plate.
480
Total Activities
158
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Pyridone amides as modulators of sodium channels
(2018)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 160 6.64 9.00
kon 160 - -
k_off 160 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3678273 3 9.00
CHEMBL3683097 3 8.70
CHEMBL3678270 3 8.52
CHEMBL3683093 3 8.30
CHEMBL3678272 3 8.22
CHEMBL3678274 3 8.15
CHEMBL3683092 3 8.00
CHEMBL3678193 3 7.96
CHEMBL5956589 3 7.96
CHEMBL3683094 3 7.96
CHEMBL3678248 3 7.92
CHEMBL6036660 3 7.89
CHEMBL3678234 3 7.89
CHEMBL3678173 3 7.85
CHEMBL3678179 3 7.85
CHEMBL3678249 3 7.80
CHEMBL3678271 3 7.80
CHEMBL3678194 6 7.77
CHEMBL3678225 3 7.75
CHEMBL3678213 3 7.72
CHEMBL3678268 3 7.72
CHEMBL3678207 3 7.70
CHEMBL5795728 3 7.62
CHEMBL3678242 3 7.58
CHEMBL3678250 3 7.58
CHEMBL3678247 3 7.57
CHEMBL3678192 3 7.55
CHEMBL3678238 3 7.54
CHEMBL3678183 3 7.52
CHEMBL3678217 3 7.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3678273 IC50 = 1.0 nM 9.00
CHEMBL3683097 IC50 = 2.0 nM 8.70
CHEMBL3678270 IC50 = 3.0 nM 8.52
CHEMBL3683093 IC50 = 5.0 nM 8.30
CHEMBL3678272 IC50 = 6.0 nM 8.22
CHEMBL3678274 IC50 = 7.0 nM 8.15
CHEMBL3683092 IC50 = 10.0 nM 8.00
CHEMBL3678193 IC50 = 11.0 nM 7.96
CHEMBL3683094 IC50 = 11.0 nM 7.96
CHEMBL5956589 IC50 = 11.0 nM 7.96
CHEMBL3678248 IC50 = 12.0 nM 7.92
CHEMBL6036660 IC50 = 13.0 nM 7.89
CHEMBL3678234 IC50 = 13.0 nM 7.89
CHEMBL3678179 IC50 = 14.0 nM 7.85
CHEMBL3678173 IC50 = 14.0 nM 7.85
CHEMBL3678249 IC50 = 16.0 nM 7.80
CHEMBL3678271 IC50 = 16.0 nM 7.80
CHEMBL3678194 IC50 = 17.0 nM 7.77
CHEMBL3678225 IC50 = 18.0 nM 7.75
CHEMBL3678213 IC50 = 19.0 nM 7.72
CHEMBL3678268 IC50 = 19.0 nM 7.72
CHEMBL3678207 IC50 = 20.0 nM 7.70
CHEMBL5795728 IC50 = 24.0 nM 7.62
CHEMBL3678250 IC50 = 26.0 nM 7.58
CHEMBL3678242 IC50 = 26.0 nM 7.58
CHEMBL3678247 IC50 = 27.0 nM 7.57
CHEMBL3678192 IC50 = 28.0 nM 7.55
CHEMBL3678238 IC50 = 29.0 nM 7.54
CHEMBL3678217 IC50 = 30.0 nM 7.52
CHEMBL3678230 IC50 = 30.0 nM 7.52
CHEMBL3678183 IC50 = 30.0 nM 7.52
CHEMBL3678239 IC50 = 31.0 nM 7.51
CHEMBL3678221 IC50 = 32.0 nM 7.50
CHEMBL3678224 IC50 = 34.0 nM 7.47
CHEMBL6032483 IC50 = 35.0 nM 7.46
CHEMBL5909547 IC50 = 36.0 nM 7.44
CHEMBL3678228 IC50 = 37.0 nM 7.43
CHEMBL3678168 IC50 = 39.0 nM 7.41
CHEMBL3678203 IC50 = 40.0 nM 7.40
CHEMBL3678191 IC50 = 44.0 nM 7.36
CHEMBL3683091 IC50 = 44.0 nM 7.36
CHEMBL3678244 IC50 = 46.0 nM 7.34
CHEMBL3678243 IC50 = 46.0 nM 7.34
CHEMBL3678216 IC50 = 47.0 nM 7.33
CHEMBL3678170 IC50 = 50.0 nM 7.30
CHEMBL3678200 IC50 = 51.0 nM 7.29
CHEMBL3678236 IC50 = 51.0 nM 7.29
CHEMBL3683095 IC50 = 52.0 nM 7.28
CHEMBL3639770 IC50 = 54.0 nM 7.27
CHEMBL3678237 IC50 = 54.0 nM 7.27