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Assay Detail

CHEMBL5733101

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Binding
hERG Binding Assay: hERG (human ether a go go-related gene) potassium channels are essential for normal electrical activity in the heart. Arrhythmia can be induced by a blockage of hERG channels by a diverse group of drugs. This side effect is a common reason for drug failure in preclinical safety trials [Sanguinetti et al., Nature, 2006, 440, 463-469.] and therefore minimisation of hERG channel blocking activity may be a desirable property for drug candidates.The purpose of the hERG binding assay is to evaluate the effects of test compounds on the voltage-dependent potassium channel encoded by the human ether go go-related gene (hERG) using a constitutively expressing CHO cell line on the Nanion Syncropatch 384PE automated patch clamp system.
381
Total Activities
126
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Chemical compounds
(2019)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 127 5.28 5.82
kon 127 - -
k_off 127 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5400637 3 5.82
CHEMBL5772929 3 5.70
CHEMBL6011841 3 5.64
CHEMBL5796450 3 5.62
CHEMBL5933859 3 5.58
CHEMBL5871237 3 5.58
CHEMBL5804138 3 5.57
CHEMBL5846746 3 5.57
CHEMBL5839498 3 5.57
CHEMBL5988920 3 5.57
CHEMBL5741828 3 5.55
CHEMBL5849595 3 5.55
CHEMBL5902781 3 5.54
CHEMBL5837180 3 5.51
CHEMBL5806518 3 5.50
CHEMBL5886475 3 5.47
CHEMBL5943322 3 5.46
CHEMBL5998681 3 5.46
CHEMBL5398790 3 5.44
CHEMBL6051199 3 5.44
CHEMBL5874010 3 5.42
CHEMBL5423660 3 5.42
CHEMBL5954593 3 5.41
CHEMBL5952392 3 5.41
CHEMBL5974706 3 5.41
CHEMBL5775436 3 5.40
CHEMBL5424734 3 5.39
CHEMBL5967894 3 5.39
CHEMBL5908705 3 5.39
CHEMBL5855181 3 5.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5400637 IC50 = 1500.0 nM 5.82
CHEMBL5772929 IC50 = 2000.0 nM 5.70
CHEMBL6011841 IC50 = 2300.0 nM 5.64
CHEMBL5796450 IC50 = 2400.0 nM 5.62
CHEMBL5871237 IC50 = 2600.0 nM 5.58
CHEMBL5933859 IC50 = 2600.0 nM 5.58
CHEMBL5988920 IC50 = 2700.0 nM 5.57
CHEMBL5839498 IC50 = 2700.0 nM 5.57
CHEMBL5804138 IC50 = 2700.0 nM 5.57
CHEMBL5846746 IC50 = 2700.0 nM 5.57
CHEMBL5741828 IC50 = 2800.0 nM 5.55
CHEMBL5849595 IC50 = 2800.0 nM 5.55
CHEMBL5902781 IC50 = 2900.0 nM 5.54
CHEMBL5837180 IC50 = 3100.0 nM 5.51
CHEMBL5806518 IC50 = 3200.0 nM 5.50
CHEMBL5886475 IC50 = 3400.0 nM 5.47
CHEMBL5998681 IC50 = 3500.0 nM 5.46
CHEMBL5943322 IC50 = 3500.0 nM 5.46
CHEMBL6051199 IC50 = 3600.0 nM 5.44
CHEMBL5398790 IC50 = 3600.0 nM 5.44
CHEMBL5423660 IC50 = 3800.0 nM 5.42
CHEMBL5874010 IC50 = 3800.0 nM 5.42
CHEMBL5952392 IC50 = 3900.0 nM 5.41
CHEMBL5974706 IC50 = 3900.0 nM 5.41
CHEMBL5954593 IC50 = 3900.0 nM 5.41
CHEMBL5775436 IC50 = 4000.0 nM 5.40
CHEMBL5967894 IC50 = 4100.0 nM 5.39
CHEMBL5855181 IC50 = 4100.0 nM 5.39
CHEMBL5424734 IC50 = 4100.0 nM 5.39
CHEMBL5908705 IC50 = 4100.0 nM 5.39
CHEMBL5972799 IC50 = 4200.0 nM 5.38
CHEMBL5743400 IC50 = 4200.0 nM 5.38
CHEMBL5975525 IC50 = 4200.0 nM 5.38
CHEMBL5927490 IC50 = 4300.0 nM 5.37
CHEMBL5926652 IC50 = 4300.0 nM 5.37
CHEMBL5978181 IC50 = 4300.0 nM 5.37
CHEMBL5788221 IC50 = 4300.0 nM 5.37
CHEMBL5400175 IC50 = 4400.0 nM 5.36
CHEMBL5912040 IC50 = 4400.0 nM 5.36
CHEMBL5407684 IC50 = 4500.0 nM 5.35
CHEMBL5432698 IC50 = 4600.0 nM 5.34
CHEMBL5760349 IC50 = 4600.0 nM 5.34
CHEMBL6007627 IC50 = 4700.0 nM 5.33
CHEMBL5969609 IC50 = 4800.0 nM 5.32
CHEMBL5934302 IC50 = 4800.0 nM 5.32
CHEMBL5752521 IC50 = 4900.0 nM 5.31
CHEMBL5832006 IC50 = 5000.0 nM 5.30
CHEMBL5834106 IC50 = 5000.0 nM 5.30
CHEMBL5775252 IC50 = 5000.0 nM 5.30
CHEMBL5421791 IC50 = 5100.0 nM 5.29