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Assay Detail

CHEMBL5733207

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Binding
FASN Inhibition: FASN activity of the SKBr3 cell extract was determined by measuring either NADPH oxidation or the amount of thiol-containing coenzyme A (CoA) released during the fatty acid synthase reaction. The dye CPM (7-diethylamino-3-(4′-maleimidyl-phenyl)-4-methylcoumarin) contains a thiol reactive group that increases its fluorescence emission on reaction with the sulfhydryl group of CoA. The biochemical activities shown in TABLE 31 were determined using the fluorescence measurement of CoA release via a procedure described in Chung C. C. et al. (Assay and Drug Development Technologies, 2008, 6(3), 361-374).
1014
Total Activities
335
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Fatty acid synthase (CHEMBL4158)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Heterocyclic modulators of lipid synthesis and combinations thereof
(2019)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 338 7.16 8.30
kon 338 - -
k_off 338 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3962197 3 8.30
CHEMBL3975955 3 8.22
CHEMBL3915660 3 8.22
CHEMBL3949918 3 8.15
CHEMBL3968803 3 8.15
CHEMBL3979428 3 8.10
CHEMBL3927497 3 8.05
CHEMBL3975941 3 8.05
CHEMBL3940149 3 8.05
CHEMBL3945625 3 8.05
CHEMBL3960666 3 8.05
CHEMBL3895704 3 8.05
CHEMBL3922048 3 8.05
CHEMBL3936614 3 8.05
CHEMBL3906251 3 8.00
CHEMBL3891794 3 8.00
CHEMBL3935073 3 8.00
CHEMBL3905299 3 8.00
CHEMBL3902171 3 7.96
CHEMBL3927676 3 7.96
CHEMBL3936277 3 7.96
CHEMBL3977044 3 7.96
CHEMBL3908611 3 7.96
CHEMBL3940947 3 7.96
CHEMBL3894044 3 7.96
CHEMBL3938270 3 7.96
CHEMBL3973206 3 7.92
CHEMBL3936004 3 7.92
CHEMBL3986980 3 7.92
CHEMBL3933162 3 7.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3962197 IC50 = 5.0 nM 8.30
CHEMBL3975955 IC50 = 6.0 nM 8.22
CHEMBL3915660 IC50 = 6.0 nM 8.22
CHEMBL3968803 IC50 = 7.0 nM 8.15
CHEMBL3949918 IC50 = 7.0 nM 8.15
CHEMBL3979428 IC50 = 8.0 nM 8.10
CHEMBL3960666 IC50 = 9.0 nM 8.05
CHEMBL3945625 IC50 = 9.0 nM 8.05
CHEMBL3936614 IC50 = 9.0 nM 8.05
CHEMBL3940149 IC50 = 9.0 nM 8.05
CHEMBL3895704 IC50 = 9.0 nM 8.05
CHEMBL3922048 IC50 = 9.0 nM 8.05
CHEMBL3975941 IC50 = 9.0 nM 8.05
CHEMBL3927497 IC50 = 9.0 nM 8.05
CHEMBL3906251 IC50 = 10.0 nM 8.00
CHEMBL3935073 IC50 = 10.0 nM 8.00
CHEMBL3905299 IC50 = 10.0 nM 8.00
CHEMBL3891794 IC50 = 10.0 nM 8.00
CHEMBL3908611 IC50 = 11.0 nM 7.96
CHEMBL3894044 IC50 = 11.0 nM 7.96
CHEMBL3977044 IC50 = 11.0 nM 7.96
CHEMBL3936277 IC50 = 11.0 nM 7.96
CHEMBL3940947 IC50 = 11.0 nM 7.96
CHEMBL3938270 IC50 = 11.0 nM 7.96
CHEMBL3902171 IC50 = 11.0 nM 7.96
CHEMBL3927676 IC50 = 11.0 nM 7.96
CHEMBL3933162 IC50 = 12.0 nM 7.92
CHEMBL3971866 IC50 = 12.0 nM 7.92
CHEMBL3973206 IC50 = 12.0 nM 7.92
CHEMBL3927639 IC50 = 12.0 nM 7.92
CHEMBL3916409 IC50 = 12.0 nM 7.92
CHEMBL3986980 IC50 = 12.0 nM 7.92
CHEMBL3936004 IC50 = 12.0 nM 7.92
CHEMBL3908686 IC50 = 13.0 nM 7.89
CHEMBL3960022 IC50 = 13.0 nM 7.89
CHEMBL3901281 IC50 = 13.0 nM 7.89
CHEMBL3893529 IC50 = 14.0 nM 7.85
CHEMBL3949269 IC50 = 14.0 nM 7.85
CHEMBL3899694 IC50 = 14.0 nM 7.85
CHEMBL3923215 IC50 = 14.0 nM 7.85
CHEMBL5839381 IC50 = 15.0 nM 7.82
CHEMBL3983146 IC50 = 15.0 nM 7.82
CHEMBL3978403 IC50 = 16.0 nM 7.80
CHEMBL3962560 IC50 = 16.0 nM 7.80
CHEMBL3971732 IC50 = 16.0 nM 7.80
CHEMBL3933878 IC50 = 16.0 nM 7.80
CHEMBL3965407 IC50 = 17.0 nM 7.77
CHEMBL3932624 IC50 = 17.0 nM 7.77
CHEMBL3919963 IC50 = 17.0 nM 7.77
CHEMBL3933609 IC50 = 18.0 nM 7.75