Assay Detail
Binding
CHEMBL5734307
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition Assay: The enzyme also can be detected based on the binding of specific ligands to the catalytic site which either immobilize the enzyme or label it with a probe such as dansyl, fluoracein, luciferase, green fluorescent protein or other reagent. The enzyme can be assayed by its hydration of EETs, its hydrolysis of an epoxide to give a colored product. The assays are normally carried out with a recombinant enzyme following affinity purification.
24
Total Activities
8
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Treatment of inflammatory disorders in non-human mammals
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 7.65 | 8.22 |
| kon | 8 | - | - |
| k_off | 8 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL242655 | — | — | 3 | 8.22 |
| CHEMBL5943959 | — | — | 3 | 8.10 |
| CHEMBL215387 | — | — | 3 | 7.96 |
| CHEMBL6041919 | — | — | 3 | 7.92 |
| CHEMBL436774 | AR9281 | 2.0 | 3 | 7.89 |
| CHEMBL1257634 | — | — | 3 | 7.22 |
| CHEMBL1076347 | TRICLOCARBAN | 2.0 | 3 | 7.21 |
| CHEMBL1668934 | — | — | 3 | 6.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL242655 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL5943959 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL215387 | — | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL6041919 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL436774 | AR9281 | IC50 | = | 13.0 | nM | 7.89 |
| CHEMBL1257634 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL1076347 | TRICLOCARBAN | IC50 | = | 61.0 | nM | 7.21 |
| CHEMBL1668934 | — | IC50 | = | 201.0 | nM | 6.70 |
| CHEMBL1076347 | TRICLOCARBAN | kon | = | - | — | - |
| CHEMBL1076347 | TRICLOCARBAN | k_off | = | - | s-1 | - |
| CHEMBL215387 | — | kon | = | - | — | - |
| CHEMBL215387 | — | k_off | = | - | s-1 | - |
| CHEMBL242655 | — | kon | = | - | — | - |
| CHEMBL242655 | — | k_off | = | - | s-1 | - |
| CHEMBL436774 | AR9281 | kon | = | - | — | - |
| CHEMBL436774 | AR9281 | k_off | = | - | s-1 | - |
| CHEMBL5943959 | — | kon | = | - | — | - |
| CHEMBL5943959 | — | k_off | = | - | s-1 | - |
| CHEMBL1668934 | — | kon | = | - | — | - |
| CHEMBL1668934 | — | k_off | = | - | s-1 | - |
| CHEMBL6041919 | — | kon | = | - | — | - |
| CHEMBL6041919 | — | k_off | = | - | s-1 | - |
| CHEMBL1257634 | — | kon | = | - | — | - |
| CHEMBL1257634 | — | k_off | = | - | s-1 | - |