Assay Detail
Binding
CHEMBL5734703
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Enzymatic Assay: Compounds were tested in an enzymatic assay using human ERK-2 (Mitogen Activated Kinase 1), recombinantly expressed as an n-terminal 6-His fusion protein in E. coli and corresponding to a 8-360. The substrate used was the fluorescent Omnia peptide S/T17 (Invitrogen of Carlsbad, Calif.; Cat. KNZ 171C). Test compounds were diluted in DMSO in 3-fold serial dilutions at 100× final concentrations. In addition to compound, the assay contained 50 mM HEPES [pH 7.3], 10 mM MgCl2, 2 mM DTT, 0.005% Triton-X100, 5 nM ERK-2 enzyme, 6.25 μM S/T17 peptide substrate and 25 μM ATP (corresponding to the observed Km) for a total reaction volume of 25 μL. The assay was run at ambient temperature in a white 384-well polypropylene plate (Nunc, Inc of Naperville, Ill.; Cat. 267462) collecting data every 50 seconds for approximately 30 minutes on an Envision plate reader (PerkinElmer, Inc. of Waltham, Mass.); Excitation 340 nm/Emission 495 nm.
330
Total Activities
106
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Serine/threonine kinase inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 110 | 9.23 | 10.70 |
| kon | 110 | - | - |
| k_off | 110 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL6045548 | — | — | 3 | 10.70 |
| CHEMBL5863069 | — | — | 3 | 10.70 |
| CHEMBL5999003 | — | — | 3 | 10.70 |
| CHEMBL5908683 | — | — | 6 | 10.70 |
| CHEMBL5806164 | — | — | 3 | 10.70 |
| CHEMBL5927076 | — | — | 3 | 10.64 |
| CHEMBL5776694 | — | — | 3 | 10.52 |
| CHEMBL5945294 | — | — | 3 | 10.52 |
| CHEMBL5887657 | — | — | 3 | 10.46 |
| CHEMBL5833636 | — | — | 3 | 10.44 |
| CHEMBL5891541 | — | — | 3 | 10.41 |
| CHEMBL5744381 | — | — | 6 | 10.14 |
| CHEMBL5876552 | — | — | 3 | 10.10 |
| CHEMBL5985409 | — | — | 3 | 9.99 |
| CHEMBL5914503 | — | — | 3 | 9.99 |
| CHEMBL5768349 | — | — | 3 | 9.96 |
| CHEMBL6055966 | — | — | 3 | 9.95 |
| CHEMBL6050192 | — | — | 3 | 9.88 |
| CHEMBL5970498 | — | — | 3 | 9.83 |
| CHEMBL5843088 | — | — | 3 | 9.83 |
| CHEMBL5959724 | — | — | 3 | 9.78 |
| CHEMBL5958147 | — | — | 3 | 9.76 |
| CHEMBL6024181 | — | — | 3 | 9.67 |
| CHEMBL5932850 | — | — | 3 | 9.56 |
| CHEMBL5904247 | — | — | 3 | 9.51 |
| CHEMBL5952404 | — | — | 3 | 9.50 |
| CHEMBL5929992 | — | — | 3 | 9.50 |
| CHEMBL5765337 | — | — | 3 | 9.42 |
| CHEMBL5988077 | — | — | 3 | 9.41 |
| CHEMBL6056771 | — | — | 3 | 9.29 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL6045548 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5863069 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5806164 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5908683 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5999003 | — | IC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5927076 | — | IC50 | = | 0.023 | nM | 10.64 |
| CHEMBL5776694 | — | IC50 | = | 0.03 | nM | 10.52 |
| CHEMBL5945294 | — | IC50 | = | 0.03 | nM | 10.52 |
| CHEMBL5887657 | — | IC50 | = | 0.035 | nM | 10.46 |
| CHEMBL5833636 | — | IC50 | = | 0.036 | nM | 10.44 |
| CHEMBL5891541 | — | IC50 | = | 0.039 | nM | 10.41 |
| CHEMBL5744381 | — | IC50 | = | 0.073 | nM | 10.14 |
| CHEMBL5876552 | — | IC50 | = | 0.079 | nM | 10.10 |
| CHEMBL5914503 | — | IC50 | = | 0.103 | nM | 9.99 |
| CHEMBL5985409 | — | IC50 | = | 0.102 | nM | 9.99 |
| CHEMBL5768349 | — | IC50 | = | 0.111 | nM | 9.96 |
| CHEMBL6055966 | — | IC50 | = | 0.113 | nM | 9.95 |
| CHEMBL6050192 | — | IC50 | = | 0.132 | nM | 9.88 |
| CHEMBL5970498 | — | IC50 | = | 0.149 | nM | 9.83 |
| CHEMBL5843088 | — | IC50 | = | 0.149 | nM | 9.83 |
| CHEMBL5959724 | — | IC50 | = | 0.168 | nM | 9.78 |
| CHEMBL5958147 | — | IC50 | = | 0.173 | nM | 9.76 |
| CHEMBL6024181 | — | IC50 | = | 0.215 | nM | 9.67 |
| CHEMBL5932850 | — | IC50 | = | 0.273 | nM | 9.56 |
| CHEMBL5904247 | — | IC50 | = | 0.311 | nM | 9.51 |
| CHEMBL5952404 | — | IC50 | = | 0.316 | nM | 9.50 |
| CHEMBL5929992 | — | IC50 | = | 0.316 | nM | 9.50 |
| CHEMBL5765337 | — | IC50 | = | 0.38 | nM | 9.42 |
| CHEMBL5988077 | — | IC50 | = | 0.39 | nM | 9.41 |
| CHEMBL6056771 | — | IC50 | = | 0.512 | nM | 9.29 |
| CHEMBL5877935 | — | IC50 | = | 0.6 | nM | 9.22 |
| CHEMBL5987978 | — | IC50 | = | 0.66 | nM | 9.18 |
| CHEMBL6044562 | — | IC50 | = | 0.655 | nM | 9.18 |
| CHEMBL5744391 | — | IC50 | = | 0.751 | nM | 9.12 |
| CHEMBL5891249 | — | IC50 | = | 1.12 | nM | 8.95 |
| CHEMBL5992303 | — | IC50 | = | 1.15 | nM | 8.94 |
| CHEMBL5764298 | — | IC50 | = | 1.17 | nM | 8.93 |
| CHEMBL5768025 | — | IC50 | = | 1.33 | nM | 8.88 |
| CHEMBL5834243 | — | IC50 | = | 1.7 | nM | 8.77 |
| CHEMBL5827057 | — | IC50 | = | 1.85 | nM | 8.73 |
| CHEMBL6039441 | — | IC50 | = | 2.3 | nM | 8.64 |
| CHEMBL5927578 | — | IC50 | = | 2.34 | nM | 8.63 |
| CHEMBL5798231 | — | IC50 | = | 2.42 | nM | 8.62 |
| CHEMBL5879549 | — | IC50 | = | 2.55 | nM | 8.59 |
| CHEMBL5879549 | — | IC50 | = | 2.55 | nM | 8.59 |
| CHEMBL5871770 | — | IC50 | = | 2.77 | nM | 8.56 |
| CHEMBL5908683 | — | IC50 | = | 2.95 | nM | 8.53 |
| CHEMBL6052694 | — | IC50 | = | 3.22 | nM | 8.49 |
| CHEMBL6011847 | — | IC50 | = | 4.28 | nM | 8.37 |
| CHEMBL5821428 | — | IC50 | = | 4.61 | nM | 8.34 |