Assay Detail
Binding
CHEMBL5735005
Review assay metadata, readout intent, target linkage, and publication context from the same page.
In Vitro Toxicity: Representative sigma-2 antagonists test compounds do not induce neuronal or glial toxicity with acute or chronic dosing in vitro. The sigma-2 receptor antagonists eliminate or reduce Abeta oligomer-induced changes in membrane trafficking. No significant effect of compounds on membrane trafficking occurs when dosed without oligomers. There is no toxicity relative to neuron number, glial number, nuclear size, nuclear morphology, neurite length, cytoskeletal morphology when tested up to 10 times the EC50 concentration for three days. See, e.g., WO2013/029060, Table 12, which is incorporated herein by reference.In vitro toxicity for Test Compounds is tested in a number of standard assays. Preferrably, testing in vitro tox studies reveals there is no genotoxicity at 10 μM (AMES, micronucleus, bacterial cytotox); HepG2 toxicity at 100-fold above affinity at sigma-2 receptor, in HepG2 tumor cell line; inhibition of CYP 450 enzymes 2D6, 3A4, and 2C19 at 10 μM; and hERG inhibition.
228
Total Activities
76
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Voltage-gated inwardly rectifying potassium channel KCNH2 (CHEMBL240) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Isoindoline compositions and methods for treating neurodegenerative disease
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 76 | 5.47 | 7.82 |
| kon | 76 | - | - |
| k_off | 76 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5928382 | — | — | 3 | 7.82 |
| CHEMBL5823135 | — | — | 3 | 7.72 |
| CHEMBL5842725 | — | — | 3 | 7.00 |
| CHEMBL5752966 | — | — | 3 | 6.89 |
| CHEMBL5975554 | — | — | 3 | 6.89 |
| CHEMBL5964118 | — | — | 3 | 6.66 |
| CHEMBL5740148 | — | — | 3 | 6.60 |
| CHEMBL4853196 | — | — | 3 | 6.54 |
| CHEMBL6030456 | — | — | 3 | 6.46 |
| CHEMBL5946331 | — | — | 3 | 6.46 |
| CHEMBL5879895 | — | — | 3 | 6.43 |
| CHEMBL6030320 | — | — | 3 | 6.37 |
| CHEMBL6051981 | — | — | 3 | 6.32 |
| CHEMBL5934082 | — | — | 3 | 6.27 |
| CHEMBL6040331 | — | — | 3 | 6.24 |
| CHEMBL5979974 | — | — | 3 | 6.18 |
| CHEMBL5964764 | — | — | 3 | 6.17 |
| CHEMBL5877899 | — | — | 3 | 6.15 |
| CHEMBL5887106 | — | — | 3 | 6.11 |
| CHEMBL5777736 | — | — | 3 | 6.10 |
| CHEMBL5841969 | — | — | 3 | 6.10 |
| CHEMBL5818991 | — | — | 3 | 6.07 |
| CHEMBL5946543 | — | — | 3 | 6.04 |
| CHEMBL6021133 | — | — | 3 | 6.01 |
| CHEMBL5783755 | — | — | 3 | 5.92 |
| CHEMBL6022823 | — | — | 3 | 5.80 |
| CHEMBL5750888 | — | — | 3 | 5.77 |
| CHEMBL6002762 | — | — | 3 | 5.72 |
| CHEMBL6010674 | — | — | 3 | 5.68 |
| CHEMBL5910884 | — | — | 3 | 5.62 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5928382 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL5823135 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL5842725 | — | IC50 | = | 99.0 | nM | 7.00 |
| CHEMBL5752966 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL5975554 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL5964118 | — | IC50 | = | 220.0 | nM | 6.66 |
| CHEMBL5740148 | — | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL4853196 | — | IC50 | = | 290.0 | nM | 6.54 |
| CHEMBL5946331 | — | IC50 | = | 350.0 | nM | 6.46 |
| CHEMBL6030456 | — | IC50 | = | 350.0 | nM | 6.46 |
| CHEMBL5879895 | — | IC50 | = | 370.0 | nM | 6.43 |
| CHEMBL6030320 | — | IC50 | = | 430.0 | nM | 6.37 |
| CHEMBL6051981 | — | IC50 | = | 480.0 | nM | 6.32 |
| CHEMBL5934082 | — | IC50 | = | 540.0 | nM | 6.27 |
| CHEMBL6040331 | — | IC50 | = | 570.0 | nM | 6.24 |
| CHEMBL5979974 | — | IC50 | = | 660.0 | nM | 6.18 |
| CHEMBL5964764 | — | IC50 | = | 670.0 | nM | 6.17 |
| CHEMBL5877899 | — | IC50 | = | 710.0 | nM | 6.15 |
| CHEMBL5887106 | — | IC50 | = | 770.0 | nM | 6.11 |
| CHEMBL5841969 | — | IC50 | = | 800.0 | nM | 6.10 |
| CHEMBL5777736 | — | IC50 | = | 790.0 | nM | 6.10 |
| CHEMBL5818991 | — | IC50 | = | 860.0 | nM | 6.07 |
| CHEMBL5946543 | — | IC50 | = | 910.0 | nM | 6.04 |
| CHEMBL6021133 | — | IC50 | = | 980.0 | nM | 6.01 |
| CHEMBL5783755 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL6022823 | — | IC50 | = | 1600.0 | nM | 5.80 |
| CHEMBL5750888 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL6002762 | — | IC50 | = | 1900.0 | nM | 5.72 |
| CHEMBL6010674 | — | IC50 | = | 2100.0 | nM | 5.68 |
| CHEMBL5910884 | — | IC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL5793914 | — | IC50 | = | 2500.0 | nM | 5.60 |
| CHEMBL5866374 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL5755473 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL5960929 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL5931677 | — | IC50 | = | 3100.0 | nM | 5.51 |
| CHEMBL5894654 | — | IC50 | = | 3200.0 | nM | 5.50 |
| CHEMBL5902857 | — | IC50 | = | 3200.0 | nM | 5.50 |
| CHEMBL6016888 | — | IC50 | = | 3200.0 | nM | 5.50 |
| CHEMBL5861338 | — | IC50 | = | 3200.0 | nM | 5.50 |
| CHEMBL6057860 | — | IC50 | = | 3700.0 | nM | 5.43 |
| CHEMBL5796179 | — | IC50 | = | 4400.0 | nM | 5.36 |
| CHEMBL5844716 | — | IC50 | = | 4700.0 | nM | 5.33 |
| CHEMBL5914083 | — | IC50 | = | 5000.0 | nM | 5.30 |
| CHEMBL5878363 | — | IC50 | = | 5100.0 | nM | 5.29 |
| CHEMBL6047079 | — | IC50 | = | 6100.0 | nM | 5.21 |
| CHEMBL5988809 | — | IC50 | = | 6600.0 | nM | 5.18 |
| CHEMBL6056346 | — | IC50 | = | 6700.0 | nM | 5.17 |
| CHEMBL4876663 | — | IC50 | = | 7000.0 | nM | 5.16 |
| CHEMBL6033554 | — | IC50 | = | 7400.0 | nM | 5.13 |
| CHEMBL4867297 | — | IC50 | = | 9600.0 | nM | 5.02 |