Assay Detail
Binding
CHEMBL5735263
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition Assay: Recombinant BACE-2 (extracellular domain, expressed in baculovirus and purified using standard methods) at 0.1 to 10 nM concentrations is incubated with the test compound at various concentrations for 1 hour at room temperature in 10 to 100 mM acetate buffer, pH 4.5, containing 0.1% CHAPS. Synthetic fluorescence-quenched peptide substrate, derived from the sequence of APP and containing a suitable fluorophore-quencher pair, is added to a final concentration of 1 to 5 μM, and the increase in fluorescence is recorded at a suitable excitation/emission wavelength in a microplate spectro-fluorimeter for 5 to 30 minutes in 1-minute intervals. IC50 values are calculated from percentage of inhibition of BACE-2 activity as a function of the test compound concentration.
144
Total Activities
45
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Heterocyclic derivatives and their use in the treatment of neurological disorders
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 48 | 6.75 | 9.00 |
| kon | 48 | - | - |
| k_off | 48 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3670372 | — | — | 3 | 9.00 |
| CHEMBL5879510 | — | — | 3 | 8.30 |
| CHEMBL3670363 | — | — | 3 | 8.30 |
| CHEMBL3670373 | — | — | 3 | 8.15 |
| CHEMBL5826565 | — | — | 3 | 8.10 |
| CHEMBL3670365 | — | — | 9 | 8.00 |
| CHEMBL3261069 | — | — | 3 | 7.92 |
| CHEMBL3670368 | — | — | 3 | 7.92 |
| CHEMBL3670366 | — | — | 3 | 7.82 |
| CHEMBL3670353 | — | — | 3 | 7.70 |
| CHEMBL5995000 | — | — | 3 | 7.62 |
| CHEMBL3670370 | — | — | 3 | 7.62 |
| CHEMBL3670375 | — | — | 3 | 7.52 |
| CHEMBL3670362 | — | — | 3 | 7.39 |
| CHEMBL3261068 | — | — | 3 | 7.26 |
| CHEMBL3670361 | — | — | 3 | 7.09 |
| CHEMBL3670355 | — | — | 6 | 7.00 |
| CHEMBL3670360 | — | — | 3 | 6.89 |
| CHEMBL3670382 | — | — | 3 | 6.89 |
| CHEMBL3670379 | — | — | 3 | 6.80 |
| CHEMBL3670381 | — | — | 3 | 6.77 |
| CHEMBL3670385 | — | — | 3 | 6.75 |
| CHEMBL5803533 | — | — | 3 | 6.62 |
| CHEMBL3670347 | — | — | 3 | 6.58 |
| CHEMBL3670371 | — | — | 3 | 6.55 |
| CHEMBL3670374 | — | — | 3 | 6.48 |
| CHEMBL3670348 | — | — | 3 | 6.44 |
| CHEMBL3670378 | — | — | 3 | 6.37 |
| CHEMBL6032553 | — | — | 3 | 6.00 |
| CHEMBL3670383 | — | — | 3 | 6.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3670372 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3670363 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5879510 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL3670373 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5826565 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3670365 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL3670368 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL3261069 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL3670366 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3670353 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL5995000 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL3670370 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL3670375 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3670362 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL3261068 | — | IC50 | = | 55.0 | nM | 7.26 |
| CHEMBL3670361 | — | IC50 | = | 82.0 | nM | 7.09 |
| CHEMBL3670355 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL3670365 | — | IC50 | = | 120.0 | nM | 6.92 |
| CHEMBL3670360 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL3670382 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL3670379 | — | IC50 | = | 160.0 | nM | 6.80 |
| CHEMBL3670381 | — | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL3670385 | — | IC50 | = | 180.0 | nM | 6.75 |
| CHEMBL5803533 | — | IC50 | = | 240.0 | nM | 6.62 |
| CHEMBL3670347 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL3670371 | — | IC50 | = | 280.0 | nM | 6.55 |
| CHEMBL3670374 | — | IC50 | = | 330.0 | nM | 6.48 |
| CHEMBL3670348 | — | IC50 | = | 360.0 | nM | 6.44 |
| CHEMBL3670378 | — | IC50 | = | 430.0 | nM | 6.37 |
| CHEMBL3670383 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL6032553 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL3670356 | — | IC50 | = | 1300.0 | nM | 5.89 |
| CHEMBL3670349 | — | IC50 | = | 1400.0 | nM | 5.85 |
| CHEMBL5756387 | — | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL3670350 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL3670365 | — | IC50 | = | 1900.0 | nM | 5.72 |
| CHEMBL3670359 | — | IC50 | = | 4200.0 | nM | 5.38 |
| CHEMBL3670357 | — | IC50 | = | 4800.0 | nM | 5.32 |
| CHEMBL5907352 | — | IC50 | = | 5400.0 | nM | 5.27 |
| CHEMBL5925547 | — | IC50 | = | 6400.0 | nM | 5.19 |
| CHEMBL3670380 | — | IC50 | = | 6500.0 | nM | 5.19 |
| CHEMBL3670352 | — | IC50 | = | 8700.0 | nM | 5.06 |
| CHEMBL3670355 | — | IC50 | = | 9900.0 | nM | 5.00 |
| CHEMBL5879510 | — | k_off | = | - | s-1 | - |
| CHEMBL3670371 | — | kon | = | - | — | - |
| CHEMBL3670375 | — | k_off | = | - | s-1 | - |
| CHEMBL3670375 | — | kon | = | - | — | - |
| CHEMBL3670374 | — | k_off | = | - | s-1 | - |
| CHEMBL3670374 | — | kon | = | - | — | - |
| CHEMBL5813321 | — | kon | = | - | — | - |