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Assay Detail

CHEMBL5735388

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
PI3K-delta Selective Assay: A compound's ability in selectively inhibiting PI3K-δ can be assessed using RAJI cells, i.e., B lymphocyte cells derived from lymphoma patients. Briefly, serum-starved RAJI cells are stimulated with anti-human IgM, thereby causing signaling through the B-cell receptors, as described in, for example, He et al., Leukemia Research (2009) 33: 798-802. B-cell receptor signaling is important for the activation, differentiation, and survival of B cells and certain B-cell derived cancers. Reduction of phospho-AKT is indicative of compounds that may inhibit B-cell proliferation and function in certain diseases. By monitoring the reduction of phospho-AKT in stimulated RAJI cells (using for example, phospho-AKT antibodies), a compound's potential efficacy in selectively inhibiting PI3Kδ can be assessed.
258
Total Activities
86
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Heterocyclic compounds and uses thereof
(2020)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 86 5.40 5.40
kon 86 - -
k_off 86 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6051763 3 5.40
CHEMBL5796536 3 5.40
CHEMBL5791000 3 5.40
CHEMBL5750806 3 5.40
CHEMBL5851176 3 5.40
CHEMBL5936480 3 5.40
CHEMBL5764062 3 5.40
CHEMBL5866941 3 5.40
CHEMBL5929894 3 5.40
CHEMBL5913203 3 5.40
CHEMBL5861424 3 5.40
CHEMBL5880726 3 5.40
CHEMBL5740069 3 5.40
CHEMBL5899739 3 5.40
CHEMBL5941496 3 5.40
CHEMBL6032645 3 5.40
CHEMBL5807905 3 5.40
CHEMBL3923629 3 5.40
CHEMBL5973275 3 5.40
CHEMBL6021741 3 5.40
CHEMBL5762937 3 5.40
CHEMBL5796587 3 5.40
CHEMBL3895629 3 5.40
CHEMBL5935134 3 5.40
CHEMBL5838632 3 5.40
CHEMBL5909018 3 5.40
CHEMBL5863742 3 5.40
CHEMBL5991752 3 5.40
CHEMBL5743380 3 5.40
CHEMBL5979518 3 5.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5898042 IC50 = 4000.0 nM 5.40
CHEMBL6041044 IC50 = 4000.0 nM 5.40
CHEMBL3955769 IC50 = 4000.0 nM 5.40
CHEMBL6063648 IC50 = 4000.0 nM 5.40
CHEMBL5741547 IC50 = 4000.0 nM 5.40
CHEMBL5829946 IC50 = 4000.0 nM 5.40
CHEMBL5965024 IC50 = 4000.0 nM 5.40
CHEMBL5935134 IC50 = 4000.0 nM 5.40
CHEMBL6059381 IC50 = 4000.0 nM 5.40
CHEMBL5740488 IC50 = 4000.0 nM 5.40
CHEMBL5889102 IC50 = 4000.0 nM 5.40
CHEMBL5923197 IC50 = 4000.0 nM 5.40
CHEMBL5867332 IC50 = 4000.0 nM 5.40
CHEMBL5838632 IC50 = 4000.0 nM 5.40
CHEMBL5922586 IC50 = 4000.0 nM 5.40
CHEMBL6015850 IC50 = 4000.0 nM 5.40
CHEMBL5992887 IC50 = 4000.0 nM 5.40
CHEMBL3895629 IC50 = 4000.0 nM 5.40
CHEMBL6013589 IC50 = 4000.0 nM 5.40
CHEMBL5913203 IC50 = 4000.0 nM 5.40
CHEMBL5953253 IC50 = 4000.0 nM 5.40
CHEMBL5929894 IC50 = 4000.0 nM 5.40
CHEMBL3928146 IC50 = 4000.0 nM 5.40
CHEMBL5790749 IC50 = 4000.0 nM 5.40
CHEMBL5798011 IC50 = 4000.0 nM 5.40
CHEMBL5900678 IC50 = 4000.0 nM 5.40
CHEMBL5991752 IC50 = 4000.0 nM 5.40
CHEMBL5971164 IC50 = 4000.0 nM 5.40
CHEMBL5974586 IC50 = 4000.0 nM 5.40
CHEMBL5743380 IC50 = 4000.0 nM 5.40
CHEMBL6011851 IC50 = 4000.0 nM 5.40
CHEMBL6053354 IC50 = 4000.0 nM 5.40
CHEMBL5860579 IC50 = 4000.0 nM 5.40
CHEMBL5979518 IC50 = 4000.0 nM 5.40
CHEMBL5863742 IC50 = 4000.0 nM 5.40
CHEMBL5789934 IC50 = 4000.0 nM 5.40
CHEMBL6021741 IC50 = 4000.0 nM 5.40
CHEMBL3893430 IC50 = 4000.0 nM 5.40
CHEMBL5900338 IC50 = 4000.0 nM 5.40
CHEMBL5935368 IC50 = 4000.0 nM 5.40
CHEMBL5933124 IC50 = 4000.0 nM 5.40
CHEMBL5909018 IC50 = 4000.0 nM 5.40
CHEMBL3914602 IC50 = 4000.0 nM 5.40
CHEMBL6007856 IC50 = 4000.0 nM 5.40
CHEMBL3946030 IC50 = 4000.0 nM 5.40
CHEMBL5910358 IC50 = 4000.0 nM 5.40
CHEMBL6050868 IC50 = 4000.0 nM 5.40
CHEMBL5745833 IC50 = 4000.0 nM 5.40
CHEMBL3921445 IC50 = 4000.0 nM 5.40
CHEMBL5900412 IC50 = 4000.0 nM 5.40