Assay Detail
Binding
CHEMBL5735587
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Fluorescence-Based Amplex Red Glutamic Acid Assay : The PSMA inhibitory activity was determined using a modification of the fluorescence-based Amplex Red Glutamic Acid Assay. Briefly, lysates of LNCaP cell extracts (25 μL) were incubated with the inhibitor (12.5 μL) in the presence of 4 μM N-acetylaspartylglutamate (NAAG) (12.5 μL) for 120 min. The amount of the glutamate released by NAAG hydrolysis was measured by incubating with a working solution (50 μL) of the Amplex Red Glutamic Acid Kit for 60 min. Fluorescence was measured with a VICTOR3V multilabel plate reader with excitation at 490 nm and emission at 642 nm. Inhibition curves were determined using semi-log plots and IC50 values were determined at the concentration at which enzyme activity was inhibited by 50%. Enzyme inhibitory constants (Ki values) were generated using the Cheng-Prusoff conversion. Assays were performed in triplicate.
192
Total Activities
35
Compounds Tested
4
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Scaffolds and multifunctional intermediates for imaging PSMA and cancer therapy
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| kon | 64 | - | - |
| k_off | 64 | - | - |
| Ki | 40 | 8.28 | 11.00 |
| IC50 | 24 | 6.78 | 8.89 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL6007312 | — | — | 3 | 11.00 |
| CHEMBL5866602 | — | — | 3 | 10.77 |
| CHEMBL5786057 | — | — | 3 | 10.70 |
| CHEMBL5994140 | — | — | 3 | 10.52 |
| CHEMBL6010838 | — | — | 3 | 10.44 |
| CHEMBL5753520 | — | — | 3 | 10.00 |
| CHEMBL5872050 | — | — | 6 | 9.59 |
| CHEMBL5838159 | — | — | 3 | 9.52 |
| CHEMBL5805978 | — | — | 9 | 9.49 |
| CHEMBL6005785 | — | — | 3 | 9.46 |
| CHEMBL5925100 | — | — | 6 | 9.40 |
| CHEMBL5916840 | — | — | 9 | 9.05 |
| CHEMBL5963808 | — | — | 3 | 8.96 |
| CHEMBL5746508 | — | — | 9 | 8.32 |
| CHEMBL5938409 | — | — | 6 | 8.22 |
| CHEMBL5937105 | — | — | 6 | 8.08 |
| CHEMBL6048554 | — | — | 6 | 8.03 |
| CHEMBL5868544 | — | — | 6 | 8.01 |
| CHEMBL5840459 | — | — | 6 | 7.92 |
| CHEMBL5975735 | — | — | 9 | 7.89 |
| CHEMBL5945455 | — | — | 3 | 7.85 |
| CHEMBL5938689 | — | — | 6 | 7.70 |
| CHEMBL5828478 | — | — | 6 | 7.68 |
| CHEMBL5778856 | — | — | 6 | 7.66 |
| CHEMBL6033575 | — | — | 6 | 7.66 |
| CHEMBL6019810 | — | — | 9 | 7.42 |
| CHEMBL5759187 | — | — | 3 | 7.38 |
| CHEMBL5960511 | — | — | 6 | 7.38 |
| CHEMBL5932131 | — | — | 6 | 6.89 |
| CHEMBL5939471 | — | — | 6 | 6.57 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL6007312 | — | Ki | = | 0.01 | nM | 11.00 |
| CHEMBL5866602 | — | Ki | = | 0.017 | nM | 10.77 |
| CHEMBL5786057 | — | Ki | = | 0.02 | nM | 10.70 |
| CHEMBL5994140 | — | Ki | = | 0.03 | nM | 10.52 |
| CHEMBL6010838 | — | Ki | = | 0.036 | nM | 10.44 |
| CHEMBL5753520 | — | Ki | = | 0.1 | nM | 10.00 |
| CHEMBL5872050 | — | Ki | = | 0.26 | nM | 9.59 |
| CHEMBL5838159 | — | Ki | = | 0.3 | nM | 9.52 |
| CHEMBL5805978 | — | Ki | = | 0.32 | nM | 9.49 |
| CHEMBL5805978 | — | Ki | = | 0.32 | nM | 9.49 |
| CHEMBL6005785 | — | Ki | = | 0.35 | nM | 9.46 |
| CHEMBL5925100 | — | Ki | = | 0.4 | nM | 9.40 |
| CHEMBL5916840 | — | Ki | = | 0.9 | nM | 9.05 |
| CHEMBL5916840 | — | Ki | = | 0.9 | nM | 9.05 |
| CHEMBL5963808 | — | Ki | = | 1.1 | nM | 8.96 |
| CHEMBL5872050 | — | IC50 | = | 1.3 | nM | 8.89 |
| CHEMBL5805978 | — | IC50 | = | 1.6 | nM | 8.80 |
| CHEMBL5925100 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL5916840 | — | IC50 | = | 4.3 | nM | 8.37 |
| CHEMBL5746508 | — | Ki | = | 4.8 | nM | 8.32 |
| CHEMBL5746508 | — | Ki | = | 4.8 | nM | 8.32 |
| CHEMBL5938409 | — | Ki | = | 6.0 | nM | 8.22 |
| CHEMBL5937105 | — | Ki | = | 8.3 | nM | 8.08 |
| CHEMBL6048554 | — | Ki | = | 9.3 | nM | 8.03 |
| CHEMBL5868544 | — | Ki | = | 9.7 | nM | 8.01 |
| CHEMBL5840459 | — | Ki | = | 12.0 | nM | 7.92 |
| CHEMBL5975735 | — | Ki | = | 13.0 | nM | 7.89 |
| CHEMBL5975735 | — | Ki | = | 13.0 | nM | 7.89 |
| CHEMBL5945455 | — | Ki | = | 14.0 | nM | 7.85 |
| CHEMBL5938689 | — | Ki | = | 20.0 | nM | 7.70 |
| CHEMBL5828478 | — | Ki | = | 21.0 | nM | 7.68 |
| CHEMBL6033575 | — | Ki | = | 22.0 | nM | 7.66 |
| CHEMBL5778856 | — | Ki | = | 22.0 | nM | 7.66 |
| CHEMBL5746508 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL5938409 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL6019810 | — | Ki | = | 38.0 | nM | 7.42 |
| CHEMBL6019810 | — | Ki | = | 38.0 | nM | 7.42 |
| CHEMBL5937105 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL5960511 | — | Ki | = | 42.0 | nM | 7.38 |
| CHEMBL5759187 | — | Ki | = | 42.0 | nM | 7.38 |
| CHEMBL6048554 | — | IC50 | = | 46.0 | nM | 7.34 |
| CHEMBL5840459 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL5975735 | — | IC50 | = | 86.0 | nM | 7.07 |
| CHEMBL5828478 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL5938689 | — | IC50 | = | 104.0 | nM | 6.98 |
| CHEMBL6033575 | — | IC50 | = | 113.0 | nM | 6.95 |
| CHEMBL5778856 | — | IC50 | = | 120.0 | nM | 6.92 |
| CHEMBL5932131 | — | Ki | = | 130.0 | nM | 6.89 |
| CHEMBL6019810 | — | IC50 | = | 192.0 | nM | 6.72 |
| CHEMBL5960511 | — | IC50 | = | 210.0 | nM | 6.68 |