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Assay Detail

CHEMBL5735590

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Binding
Electrophysiology Assay: Patch clamp electrophysiology was used to assess the efficacy and selectivity of sodium channel blockers in dorsal root ganglion neurons. Rat neurons were isolated from the dorsal root ganglions and maintained in culture for 2 to 10 days in the presence of NGF (50 ng/ml) (culture media consisted of NeurobasalA supplemented with B27, glutamine and antibiotics). Small diameter neurons (nociceptors, 8-12 μm in diameter) were visually identified and probed with fine tip glass electrodes connected to an amplifier (Axon Instruments). The “voltage clamp” mode was used to assess the compound's IC50 holding the cells at −60 mV. In addition, the “current clamp” mode was employed to test the efficacy of the compounds in blocking action potential generation in response to current injections. The results of these experiments contributed to the definition of the efficacy profile of the compounds.
480
Total Activities
158
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Pyridone amides as modulators of sodium channels
(2020)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 160 6.64 9.00
kon 160 - -
k_off 160 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3678273 3 9.00
CHEMBL3683097 3 8.70
CHEMBL3678270 3 8.52
CHEMBL6025436 3 8.30
CHEMBL3678272 3 8.22
CHEMBL3678274 3 8.15
CHEMBL3683092 3 8.00
CHEMBL3678193 6 7.96
CHEMBL3678240 3 7.96
CHEMBL3683094 3 7.96
CHEMBL3678248 3 7.92
CHEMBL3678232 3 7.89
CHEMBL3678234 3 7.89
CHEMBL3678173 3 7.85
CHEMBL3678179 3 7.85
CHEMBL3678249 3 7.80
CHEMBL3678271 3 7.80
CHEMBL3678194 6 7.77
CHEMBL5864294 3 7.75
CHEMBL3678213 3 7.72
CHEMBL3678268 3 7.72
CHEMBL3678207 3 7.70
CHEMBL5979106 3 7.62
CHEMBL5752396 3 7.58
CHEMBL3678250 3 7.58
CHEMBL5839580 3 7.57
CHEMBL3678192 3 7.55
CHEMBL3678238 3 7.54
CHEMBL3678183 3 7.52
CHEMBL6063162 3 7.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3678273 IC50 = 1.0 nM 9.00
CHEMBL3683097 IC50 = 2.0 nM 8.70
CHEMBL3678270 IC50 = 3.0 nM 8.52
CHEMBL6025436 IC50 = 5.0 nM 8.30
CHEMBL3678272 IC50 = 6.0 nM 8.22
CHEMBL3678274 IC50 = 7.0 nM 8.15
CHEMBL3683092 IC50 = 10.0 nM 8.00
CHEMBL3678193 IC50 = 11.0 nM 7.96
CHEMBL3683094 IC50 = 11.0 nM 7.96
CHEMBL3678240 IC50 = 11.0 nM 7.96
CHEMBL3678248 IC50 = 12.0 nM 7.92
CHEMBL3678232 IC50 = 13.0 nM 7.89
CHEMBL3678234 IC50 = 13.0 nM 7.89
CHEMBL3678179 IC50 = 14.0 nM 7.85
CHEMBL3678173 IC50 = 14.0 nM 7.85
CHEMBL3678249 IC50 = 16.0 nM 7.80
CHEMBL3678271 IC50 = 16.0 nM 7.80
CHEMBL3678194 IC50 = 17.0 nM 7.77
CHEMBL5864294 IC50 = 18.0 nM 7.75
CHEMBL3678213 IC50 = 19.0 nM 7.72
CHEMBL3678268 IC50 = 19.0 nM 7.72
CHEMBL3678207 IC50 = 20.0 nM 7.70
CHEMBL5979106 IC50 = 24.0 nM 7.62
CHEMBL3678250 IC50 = 26.0 nM 7.58
CHEMBL5752396 IC50 = 26.0 nM 7.58
CHEMBL5839580 IC50 = 27.0 nM 7.57
CHEMBL3678192 IC50 = 28.0 nM 7.55
CHEMBL3678238 IC50 = 29.0 nM 7.54
CHEMBL6063162 IC50 = 30.0 nM 7.52
CHEMBL3678230 IC50 = 30.0 nM 7.52
CHEMBL3678183 IC50 = 30.0 nM 7.52
CHEMBL3678239 IC50 = 31.0 nM 7.51
CHEMBL6034756 IC50 = 32.0 nM 7.50
CHEMBL3678224 IC50 = 34.0 nM 7.47
CHEMBL6032483 IC50 = 35.0 nM 7.46
CHEMBL5909547 IC50 = 36.0 nM 7.44
CHEMBL3678228 IC50 = 37.0 nM 7.43
CHEMBL3678168 IC50 = 39.0 nM 7.41
CHEMBL3678203 IC50 = 40.0 nM 7.40
CHEMBL3678191 IC50 = 44.0 nM 7.36
CHEMBL3683091 IC50 = 44.0 nM 7.36
CHEMBL3678244 IC50 = 46.0 nM 7.34
CHEMBL3678243 IC50 = 46.0 nM 7.34
CHEMBL5858227 IC50 = 47.0 nM 7.33
CHEMBL3678170 IC50 = 50.0 nM 7.30
CHEMBL3678200 IC50 = 51.0 nM 7.29
CHEMBL3678236 IC50 = 51.0 nM 7.29
CHEMBL3683095 IC50 = 52.0 nM 7.28
CHEMBL3639770 IC50 = 54.0 nM 7.27
CHEMBL5992932 IC50 = 54.0 nM 7.27