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Assay Detail

CHEMBL5736331

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Binding
Biomol Green Assay: Biomol Green (Enzo) is a bioluminescent, homogeneous assay that measures ADP formed from a biochemical reaction. Because of its high sensitivity, the assay is suitable for monitoring enzyme activities at very early substrate conversions requiring very low amount of enzymes. This is critical since inhibitor potency has to be demonstrated at the cellular level where ATP is present at millimolar concentrations. The assay procedure used may be the same as in Zhang et al., “Altered cofactor regulation with disease-associated p97/VCP mutations,” Proc. Natl. Acad. Sci. USA, 112(14), E1705-E1714 (2015).
ChEMBL ↗
396
Total Activities
120
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545) ↗
Type UNCHECKED

Publication

Modulators of P97 AAA ATPase activity
(2021)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 132 7.15 8.15
kon 132 - -
k_off 132 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4450581 — — 3 8.15
CHEMBL5499040 — — 3 8.00
CHEMBL5987600 — — 3 7.96
CHEMBL5416704 — — 3 7.92
CHEMBL5399073 — — 3 7.92
CHEMBL5402777 — — 6 7.89
CHEMBL5768570 — — 6 7.82
CHEMBL5824089 — — 3 7.80
CHEMBL5818234 — — 3 7.80
CHEMBL5419713 — — 3 7.77
CHEMBL6028853 — — 9 7.72
CHEMBL5942032 — — 3 7.72
CHEMBL6057814 — — 3 7.70
CHEMBL5805570 — — 3 7.70
CHEMBL5914612 — — 3 7.68
CHEMBL6024379 — — 3 7.68
CHEMBL5921555 — — 3 7.64
CHEMBL6041382 — — 6 7.60
CHEMBL5414510 — — 3 7.60
CHEMBL5413736 — — 6 7.60
CHEMBL5876499 — — 3 7.58
CHEMBL5910572 — — 3 7.58
CHEMBL5823812 — — 3 7.58
CHEMBL5934978 — — 3 7.58
CHEMBL5787392 — — 6 7.57
CHEMBL5948191 — — 9 7.55
CHEMBL6014832 — — 3 7.54
CHEMBL5867846 — — 3 7.54
CHEMBL5409286 — — 3 7.52
CHEMBL5940749 — — 3 7.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4450581 — IC50 = 7.0 nM 8.15
CHEMBL5499040 — IC50 = 10.0 nM 8.00
CHEMBL5987600 — IC50 = 11.0 nM 7.96
CHEMBL5416704 — IC50 = 12.0 nM 7.92
CHEMBL5399073 — IC50 = 12.0 nM 7.92
CHEMBL5402777 — IC50 = 13.0 nM 7.89
CHEMBL5768570 — IC50 = 15.0 nM 7.82
CHEMBL5818234 — IC50 = 16.0 nM 7.80
CHEMBL5824089 — IC50 = 16.0 nM 7.80
CHEMBL5419713 — IC50 = 17.0 nM 7.77
CHEMBL6028853 — IC50 = 19.0 nM 7.72
CHEMBL6028853 — IC50 = 19.0 nM 7.72
CHEMBL5942032 — IC50 = 19.0 nM 7.72
CHEMBL5805570 — IC50 = 20.0 nM 7.70
CHEMBL6057814 — IC50 = 20.0 nM 7.70
CHEMBL5402777 — IC50 = 20.0 nM 7.70
CHEMBL6024379 — IC50 = 21.0 nM 7.68
CHEMBL5914612 — IC50 = 21.0 nM 7.68
CHEMBL5921555 — IC50 = 23.0 nM 7.64
CHEMBL6041382 — IC50 = 25.0 nM 7.60
CHEMBL5768570 — IC50 = 25.0 nM 7.60
CHEMBL5414510 — IC50 = 25.0 nM 7.60
CHEMBL5413736 — IC50 = 25.0 nM 7.60
CHEMBL5823812 — IC50 = 26.0 nM 7.58
CHEMBL5910572 — IC50 = 26.0 nM 7.58
CHEMBL5934978 — IC50 = 26.0 nM 7.58
CHEMBL5876499 — IC50 = 26.0 nM 7.58
CHEMBL5787392 — IC50 = 27.0 nM 7.57
CHEMBL5948191 — IC50 = 28.0 nM 7.55
CHEMBL5867846 — IC50 = 29.0 nM 7.54
CHEMBL6014832 — IC50 = 29.0 nM 7.54
CHEMBL5409286 — IC50 = 30.0 nM 7.52
CHEMBL6067937 — IC50 = 30.0 nM 7.52
CHEMBL5879020 — IC50 = 30.0 nM 7.52
CHEMBL5879020 — IC50 = 30.0 nM 7.52
CHEMBL6038448 — IC50 = 30.0 nM 7.52
CHEMBL5413736 — IC50 = 30.0 nM 7.52
CHEMBL5940749 — IC50 = 30.0 nM 7.52
CHEMBL5777115 — IC50 = 30.0 nM 7.52
CHEMBL5984170 — IC50 = 30.0 nM 7.52
CHEMBL5827577 — IC50 = 30.0 nM 7.52
CHEMBL5787392 — IC50 = 32.0 nM 7.50
CHEMBL5975446 — IC50 = 32.0 nM 7.50
CHEMBL6041382 — IC50 = 34.0 nM 7.47
CHEMBL5779612 — IC50 = 34.0 nM 7.47
CHEMBL5404554 — IC50 = 35.0 nM 7.46
CHEMBL5806424 — IC50 = 35.0 nM 7.46
CHEMBL5419453 — IC50 = 36.0 nM 7.44
CHEMBL5426808 — IC50 = 37.0 nM 7.43
CHEMBL6038108 — IC50 = 38.0 nM 7.42