Assay Detail
Binding
CHEMBL5736349
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Fluorescence Polarization (FP) Competitive Binding Assay: A fluorescence polarization (FP) competitive binding assay was used to determine the binding affinities of representative menin inhibitors. A FAM labeled fluorescent probe was designed and synthesized based on a MLL1 peptide (FAM-MM2). Equilibrium dissociation constant (Kd) value of FAM-MM2 to menin protein was determined from protein saturation experiments by monitoring the total fluorescence polarization of mixtures composed with the fluorescent probe at a fixed concentration and the protein with increasing concentrations up to full saturation. Serial dilutions of the protein were mixed with FAM-MM2 to a final volume of 200 μl in the assay buffer (PBS with 0.02% Bovine γ-Globulin and 4% DMSO. 0.01% Triton X-100 was added right before assays). Final FAM-MM2 concentration was 2 nM. Plates were incubated at room temperature for 30 minutes with gentle shaking to assure equilibrium. FP values in millipolarization units (mP) were measured using the Infinite M-1000 plate reader (Tecan U.S., Research Triangle Park, N.C.) in Microfluor 1 96-well, black, v-bottom plates (Thermo Scientific, Waltham, Mash.) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. Kd value of FAM-MM2, which was calculated by fitting the sigmoidal dose-dependent FP increases as a function of protein concentrations using Graphpad Prism 6.0 software (Graphpad Software, San Diego, Calif.), is determined as 1.4 nM.
1158
Total Activities
378
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Piperidines as menin inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 386 | 6.53 | 8.52 |
| kon | 386 | - | - |
| k_off | 386 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5816906 | — | — | 3 | 8.52 |
| CHEMBL5974610 | — | — | 3 | 8.52 |
| CHEMBL5905647 | — | — | 3 | 8.52 |
| CHEMBL5931080 | — | — | 3 | 8.52 |
| CHEMBL5964939 | — | — | 3 | 8.52 |
| CHEMBL6047118 | — | — | 3 | 8.52 |
| CHEMBL5798441 | — | — | 3 | 8.40 |
| CHEMBL5829566 | — | — | 3 | 8.40 |
| CHEMBL5938942 | — | — | 3 | 8.40 |
| CHEMBL5975630 | — | — | 3 | 8.40 |
| CHEMBL5822575 | — | — | 3 | 8.40 |
| CHEMBL5985246 | — | — | 3 | 8.40 |
| CHEMBL5743156 | — | — | 3 | 8.40 |
| CHEMBL6020537 | — | — | 3 | 8.30 |
| CHEMBL5948342 | — | — | 3 | 8.30 |
| CHEMBL5960556 | — | — | 3 | 8.30 |
| CHEMBL6013737 | — | — | 6 | 8.30 |
| CHEMBL4585198 | — | — | 3 | 8.22 |
| CHEMBL5790028 | — | — | 3 | 8.22 |
| CHEMBL5783513 | — | — | 3 | 8.22 |
| CHEMBL5767175 | — | — | 3 | 8.22 |
| CHEMBL6034357 | — | — | 3 | 8.22 |
| CHEMBL5893927 | — | — | 3 | 8.22 |
| CHEMBL4551865 | — | — | 3 | 8.15 |
| CHEMBL4592636 | — | — | 3 | 8.15 |
| CHEMBL5843849 | — | — | 3 | 8.15 |
| CHEMBL5769793 | — | — | 3 | 8.15 |
| CHEMBL5777937 | — | — | 3 | 8.15 |
| CHEMBL5859938 | — | — | 3 | 8.15 |
| CHEMBL5783978 | — | — | 3 | 8.15 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5905647 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL5931080 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL5816906 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL5974610 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL5964939 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL6047118 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL5743156 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5798441 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5938942 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5822575 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5985246 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5829566 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5975630 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL6013737 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5948342 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5960556 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL6020537 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL5790028 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4585198 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL6034357 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL5783513 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL5767175 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL5893927 | — | IC50 | = | 6.0 | nM | 8.22 |
| CHEMBL4551865 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5859938 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5777937 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5769793 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5935610 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5783978 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL4592636 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5899857 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5843849 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL5936667 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5962223 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5788808 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5893826 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL6029944 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5769865 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5836021 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5820786 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5848603 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL4449199 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL5835328 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5794080 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL4522767 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5865073 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5768069 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5765474 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5970007 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL5740449 | — | IC50 | = | 9.0 | nM | 8.05 |