Assay Detail
Binding
CHEMBL5737050
Review assay metadata, readout intent, target linkage, and publication context from the same page.
fluorescence anisotropy assay: The affinity of Example 1-33 for galectins were determined by a fluorescence anisotropy assay where the compound was used as an inhibitor of the interaction between galectin and a fluorescein tagged saccharide probe as described Sörme, P., Kahl-Knutsson, B., Huflejt, M., Nilsson, U. J., and Leffler H. (2004) Fluorescence polarization as an analytical tool to evaluate galectin-ligand interactions. Anal. Biochem. 334: 36-47, (Sörme et al., 2004) and Monovalent interactions of Galectin-1 By Salomonsson, Emma; Larumbe, Amaia; Tejler, Johan; Tullberg, Erik; Rydberg, Hanna; Sundin, Anders; Khabut, Areej; Frejd, Torbjorn; Lobsanov, Yuri D.; Rini, James M.; et al, From Biochemistry (2010), 49(44), 9518-9532, (Salomonsson et al., 2010).
78
Total Activities
26
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Galactoside inhibitor of galectins
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 26 | 5.74 | 7.16 |
| kon | 26 | - | - |
| k_off | 26 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5849127 | — | — | 3 | 7.16 |
| CHEMBL5746410 | — | — | 3 | 6.70 |
| CHEMBL5837954 | — | — | 3 | 6.66 |
| CHEMBL5859811 | — | — | 3 | 6.60 |
| CHEMBL5777987 | — | — | 3 | 6.60 |
| CHEMBL5871228 | — | — | 3 | 6.38 |
| CHEMBL5850132 | — | — | 3 | 6.24 |
| CHEMBL5807477 | — | — | 3 | 5.85 |
| CHEMBL5954468 | — | — | 3 | 5.77 |
| CHEMBL5993674 | — | — | 3 | 5.77 |
| CHEMBL5978139 | — | — | 3 | 5.68 |
| CHEMBL5866001 | — | — | 3 | 5.66 |
| CHEMBL5950915 | — | — | 3 | 5.58 |
| CHEMBL5890322 | — | — | 3 | 5.55 |
| CHEMBL5829222 | — | — | 3 | 5.55 |
| CHEMBL5790628 | — | — | 3 | 5.51 |
| CHEMBL5772323 | — | — | 3 | 5.34 |
| CHEMBL5925466 | — | — | 3 | 5.34 |
| CHEMBL5929368 | — | — | 3 | 5.24 |
| CHEMBL5809155 | — | — | 3 | 5.22 |
| CHEMBL5966583 | — | — | 3 | 5.22 |
| CHEMBL5865693 | — | — | 3 | 5.19 |
| CHEMBL5887621 | — | — | 3 | 5.18 |
| CHEMBL5809066 | — | — | 3 | 5.10 |
| CHEMBL5858997 | — | — | 3 | 5.07 |
| CHEMBL5921738 | — | — | 3 | 5.01 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5849127 | — | Kd | = | 69.0 | nM | 7.16 |
| CHEMBL5746410 | — | Kd | = | 200.0 | nM | 6.70 |
| CHEMBL5837954 | — | Kd | = | 220.0 | nM | 6.66 |
| CHEMBL5777987 | — | Kd | = | 250.0 | nM | 6.60 |
| CHEMBL5859811 | — | Kd | = | 250.0 | nM | 6.60 |
| CHEMBL5871228 | — | Kd | = | 420.0 | nM | 6.38 |
| CHEMBL5850132 | — | Kd | = | 580.0 | nM | 6.24 |
| CHEMBL5807477 | — | Kd | = | 1400.0 | nM | 5.85 |
| CHEMBL5954468 | — | Kd | = | 1700.0 | nM | 5.77 |
| CHEMBL5993674 | — | Kd | = | 1700.0 | nM | 5.77 |
| CHEMBL5978139 | — | Kd | = | 2100.0 | nM | 5.68 |
| CHEMBL5866001 | — | Kd | = | 2200.0 | nM | 5.66 |
| CHEMBL5950915 | — | Kd | = | 2600.0 | nM | 5.58 |
| CHEMBL5890322 | — | Kd | = | 2800.0 | nM | 5.55 |
| CHEMBL5829222 | — | Kd | = | 2800.0 | nM | 5.55 |
| CHEMBL5790628 | — | Kd | = | 3100.0 | nM | 5.51 |
| CHEMBL5925466 | — | Kd | = | 4600.0 | nM | 5.34 |
| CHEMBL5772323 | — | Kd | = | 4600.0 | nM | 5.34 |
| CHEMBL5929368 | — | Kd | = | 5700.0 | nM | 5.24 |
| CHEMBL5966583 | — | Kd | = | 6000.0 | nM | 5.22 |
| CHEMBL5809155 | — | Kd | = | 6000.0 | nM | 5.22 |
| CHEMBL5865693 | — | Kd | = | 6500.0 | nM | 5.19 |
| CHEMBL5887621 | — | Kd | = | 6600.0 | nM | 5.18 |
| CHEMBL5809066 | — | Kd | = | 8000.0 | nM | 5.10 |
| CHEMBL5858997 | — | Kd | = | 8600.0 | nM | 5.07 |
| CHEMBL5921738 | — | Kd | = | 9700.0 | nM | 5.01 |
| CHEMBL5929368 | — | kon | = | - | — | - |
| CHEMBL5777987 | — | k_off | = | - | s-1 | - |
| CHEMBL5777987 | — | kon | = | - | — | - |
| CHEMBL5993674 | — | k_off | = | - | s-1 | - |
| CHEMBL5954468 | — | kon | = | - | — | - |
| CHEMBL5978139 | — | k_off | = | - | s-1 | - |
| CHEMBL5978139 | — | kon | = | - | — | - |
| CHEMBL5925466 | — | k_off | = | - | s-1 | - |
| CHEMBL5925466 | — | kon | = | - | — | - |
| CHEMBL5849127 | — | k_off | = | - | s-1 | - |
| CHEMBL5849127 | — | kon | = | - | — | - |
| CHEMBL5929368 | — | k_off | = | - | s-1 | - |
| CHEMBL5772323 | — | k_off | = | - | s-1 | - |
| CHEMBL5887621 | — | k_off | = | - | s-1 | - |
| CHEMBL5887621 | — | kon | = | - | — | - |
| CHEMBL5865693 | — | k_off | = | - | s-1 | - |
| CHEMBL5865693 | — | kon | = | - | — | - |
| CHEMBL5790628 | — | k_off | = | - | s-1 | - |
| CHEMBL5809155 | — | k_off | = | - | s-1 | - |
| CHEMBL5954468 | — | k_off | = | - | s-1 | - |
| CHEMBL5790628 | — | kon | = | - | — | - |
| CHEMBL5772323 | — | kon | = | - | — | - |
| CHEMBL5966583 | — | kon | = | - | — | - |
| CHEMBL5966583 | — | k_off | = | - | s-1 | - |