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Assay Detail

CHEMBL5737829

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Binding
Biochemical Assay: Test compounds were placed in a Greiner Bio-One (Monroe, N.C.) 1536-well black solid bottom assay plate. 200 millimolar (mM) Tris HCl, pH 7.4, 100 micromolar (μM) EDTA and 0.01% TWEEN-20™, final concentration, was used as the assay buffer. The LDHA reagent was 2 nanomolar (nM) Human LDHA (Meridian Life Science, Inc., Memphis, Tenn.), final concentration, in assay buffer. The substrate reagent was 0.06 mM NADH and 0.2 mM sodium pyruvate, final concentration, in assay buffer. The resazurin/diaphorase coupling reagent was 0.037 mM resazurin and 0.133 milligrams per milliliter (mg/mL) diaphorase, final concentration, in assay buffer.
1566
Total Activities
510
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Small molecule inhibitors of lactate dehydrogenase and methods of use thereof
(2022)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 522 5.11 6.26
kon 522 - -
k_off 522 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4066429 3 6.26
CHEMBL5906632 3 6.26
CHEMBL5817226 3 6.26
CHEMBL5756621 3 6.26
CHEMBL5927493 3 6.26
CHEMBL5747101 3 6.26
CHEMBL5908466 3 6.26
CHEMBL6053543 3 6.26
CHEMBL5914170 3 6.26
CHEMBL5821897 3 6.26
CHEMBL5970507 3 6.26
CHEMBL5824084 3 6.26
CHEMBL5762971 3 6.26
CHEMBL5861592 3 6.26
CHEMBL5891480 3 6.26
CHEMBL5967853 3 6.26
CHEMBL4089051 3 6.26
CHEMBL6022710 3 6.26
CHEMBL5809745 3 6.26
CHEMBL5919037 3 6.26
CHEMBL5984996 6 6.26
CHEMBL5859497 3 6.26
CHEMBL5799758 3 6.26
CHEMBL4099443 3 6.26
CHEMBL4063125 3 6.26
CHEMBL4076973 6 6.26
CHEMBL5896240 3 6.26
CHEMBL5994969 3 6.26
CHEMBL6045830 3 6.26
CHEMBL6064754 3 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6023002 IC50 = 550.0 nM 6.26
CHEMBL5994969 IC50 = 550.0 nM 6.26
CHEMBL5970507 IC50 = 550.0 nM 6.26
CHEMBL5889775 IC50 = 550.0 nM 6.26
CHEMBL4066429 IC50 = 550.0 nM 6.26
CHEMBL5914464 IC50 = 550.0 nM 6.26
CHEMBL5838640 IC50 = 550.0 nM 6.26
CHEMBL6045830 IC50 = 550.0 nM 6.26
CHEMBL4763906 IC50 = 550.0 nM 6.26
CHEMBL4790696 IC50 = 550.0 nM 6.26
CHEMBL5972786 IC50 = 550.0 nM 6.26
CHEMBL5919037 IC50 = 550.0 nM 6.26
CHEMBL5799758 IC50 = 550.0 nM 6.26
CHEMBL5773388 IC50 = 550.0 nM 6.26
CHEMBL5967853 IC50 = 550.0 nM 6.26
CHEMBL5821897 IC50 = 550.0 nM 6.26
CHEMBL4089051 IC50 = 550.0 nM 6.26
CHEMBL4076973 IC50 = 550.0 nM 6.26
CHEMBL5914170 IC50 = 550.0 nM 6.26
CHEMBL5977606 IC50 = 550.0 nM 6.26
CHEMBL6064754 IC50 = 550.0 nM 6.26
CHEMBL5762971 IC50 = 550.0 nM 6.26
CHEMBL4076973 IC50 = 550.0 nM 6.26
CHEMBL6053543 IC50 = 550.0 nM 6.26
CHEMBL5809745 IC50 = 550.0 nM 6.26
CHEMBL5755709 IC50 = 550.0 nM 6.26
CHEMBL5867475 IC50 = 550.0 nM 6.26
CHEMBL5958891 IC50 = 550.0 nM 6.26
CHEMBL5891389 IC50 = 550.0 nM 6.26
CHEMBL5824084 IC50 = 550.0 nM 6.26
CHEMBL5818031 IC50 = 550.0 nM 6.26
CHEMBL5977404 IC50 = 550.0 nM 6.26
CHEMBL5927493 IC50 = 550.0 nM 6.26
CHEMBL5890901 IC50 = 550.0 nM 6.26
CHEMBL5861613 IC50 = 550.0 nM 6.26
CHEMBL4864038 IC50 = 550.0 nM 6.26
CHEMBL5974320 IC50 = 550.0 nM 6.26
CHEMBL5947721 IC50 = 550.0 nM 6.26
CHEMBL5984996 IC50 = 550.0 nM 6.26
CHEMBL5756621 IC50 = 550.0 nM 6.26
CHEMBL5891480 IC50 = 550.0 nM 6.26
CHEMBL5747101 IC50 = 550.0 nM 6.26
CHEMBL5841942 IC50 = 550.0 nM 6.26
CHEMBL5858423 IC50 = 550.0 nM 6.26
CHEMBL6035886 IC50 = 550.0 nM 6.26
CHEMBL5817226 IC50 = 550.0 nM 6.26
CHEMBL5896240 IC50 = 550.0 nM 6.26
CHEMBL5908466 IC50 = 550.0 nM 6.26
CHEMBL5834609 IC50 = 550.0 nM 6.26
CHEMBL5826765 IC50 = 550.0 nM 6.26