Assay Detail
Binding
CHEMBL5738652
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Radioligand Binding Assay: Each compound was initially tested at 20 μM and was incubated with 3H-DPN at a concentration equal to the Kd (0.4 nM) of the radioligand in μOR containing Sf9 insect cell membranes. The reaction contained 40 fmol of μOR and was incubated in a buffer of 20 mM HEPES pH 7.5, 100 mM sodium chloride, and 0.1% bovine serum albumin for 1 hour at 25° C. To separate free from bound radioligand, reactions were rapidly filtered over Whatman GF/B filters with the aid of a Brandel harvester and 3H-DPN counts were measured by liquid scintillation. Compounds with more than 25% of 3H-DPN radioactivity were further tested in full dose-response to determine the affinity (Ki) in HEK293 membranes. Subsequently, the 15 analogs were tested in full dose-response for affinity at the μOR and the κOR by the National Institutes of Mental Health Psychoactive Drug Screen Program (PDSP), as were the affinities of compounds 12, PZM21, and their stereoisomers at the μOR, δOR, κOR and nociception receptor.
264
Total Activities
64
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Mu opioid receptor modulators
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 88 | 6.63 | 9.03 |
| kon | 88 | - | - |
| k_off | 88 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5852351 | — | — | 6 | 9.03 |
| CHEMBL5776103 | — | — | 6 | 8.43 |
| CHEMBL5776546 | — | — | 6 | 8.43 |
| CHEMBL5853846 | — | — | 3 | 8.35 |
| CHEMBL5882320 | — | — | 6 | 8.33 |
| CHEMBL5929440 | — | — | 6 | 8.25 |
| CHEMBL5908700 | — | — | 6 | 8.23 |
| CHEMBL5998144 | — | — | 3 | 8.19 |
| CHEMBL5745275 | — | — | 3 | 7.92 |
| CHEMBL4467777 | — | — | 6 | 7.75 |
| CHEMBL5803638 | — | — | 6 | 7.72 |
| CHEMBL6039799 | — | — | 3 | 7.66 |
| CHEMBL5988995 | — | — | 3 | 7.57 |
| CHEMBL5972109 | — | — | 3 | 7.50 |
| CHEMBL6032981 | — | — | 6 | 7.50 |
| CHEMBL6009475 | — | — | 6 | 7.47 |
| CHEMBL5399232 | — | — | 3 | 7.46 |
| CHEMBL5850302 | — | — | 3 | 7.43 |
| CHEMBL5882410 | — | — | 3 | 7.35 |
| CHEMBL6055529 | — | — | 6 | 7.34 |
| CHEMBL5987534 | — | — | 3 | 7.34 |
| CHEMBL5797861 | — | — | 6 | 7.33 |
| CHEMBL5889040 | — | — | 3 | 7.29 |
| CHEMBL5848559 | — | — | 3 | 7.22 |
| CHEMBL5942121 | — | — | 3 | 7.17 |
| CHEMBL5907496 | — | — | 3 | 7.13 |
| CHEMBL5878755 | — | — | 3 | 7.07 |
| CHEMBL6039109 | — | — | 3 | 7.07 |
| CHEMBL5795210 | — | — | 6 | 7.04 |
| CHEMBL5892025 | — | — | 3 | 7.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5852351 | — | Ki | = | 0.93 | nM | 9.03 |
| CHEMBL5776546 | — | Ki | = | 3.7 | nM | 8.43 |
| CHEMBL5776103 | — | Ki | = | 3.7 | nM | 8.43 |
| CHEMBL5853846 | — | Ki | = | 4.5 | nM | 8.35 |
| CHEMBL5882320 | — | Ki | = | 4.7 | nM | 8.33 |
| CHEMBL5929440 | — | Ki | = | 5.6 | nM | 8.25 |
| CHEMBL5908700 | — | Ki | = | 5.9 | nM | 8.23 |
| CHEMBL5998144 | — | Ki | = | 6.5 | nM | 8.19 |
| CHEMBL5745275 | — | Ki | = | 12.0 | nM | 7.92 |
| CHEMBL4467777 | — | Ki | = | 18.0 | nM | 7.75 |
| CHEMBL5803638 | — | Ki | = | 19.0 | nM | 7.72 |
| CHEMBL6039799 | — | Ki | = | 22.0 | nM | 7.66 |
| CHEMBL5776103 | — | Ki | = | 23.0 | nM | 7.64 |
| CHEMBL5988995 | — | Ki | = | 27.0 | nM | 7.57 |
| CHEMBL6032981 | — | Ki | = | 32.0 | nM | 7.50 |
| CHEMBL5972109 | — | Ki | = | 32.0 | nM | 7.50 |
| CHEMBL6009475 | — | Ki | = | 34.0 | nM | 7.47 |
| CHEMBL5399232 | — | Ki | = | 35.0 | nM | 7.46 |
| CHEMBL5850302 | — | Ki | = | 37.0 | nM | 7.43 |
| CHEMBL5882410 | — | Ki | = | 45.0 | nM | 7.35 |
| CHEMBL4467777 | — | Ki | = | 46.0 | nM | 7.34 |
| CHEMBL5987534 | — | Ki | = | 46.0 | nM | 7.34 |
| CHEMBL6055529 | — | Ki | = | 46.0 | nM | 7.34 |
| CHEMBL5797861 | — | Ki | = | 47.0 | nM | 7.33 |
| CHEMBL5889040 | — | Ki | = | 51.0 | nM | 7.29 |
| CHEMBL5848559 | — | Ki | = | 60.0 | nM | 7.22 |
| CHEMBL5803638 | — | Ki | = | 67.0 | nM | 7.17 |
| CHEMBL5942121 | — | Ki | = | 67.0 | nM | 7.17 |
| CHEMBL5907496 | — | Ki | = | 74.0 | nM | 7.13 |
| CHEMBL5878755 | — | Ki | = | 86.0 | nM | 7.07 |
| CHEMBL6039109 | — | Ki | = | 86.0 | nM | 7.07 |
| CHEMBL5795210 | — | Ki | = | 91.0 | nM | 7.04 |
| CHEMBL5892025 | — | Ki | = | 100.0 | nM | 7.00 |
| CHEMBL5997271 | — | Ki | = | 140.0 | nM | 6.85 |
| CHEMBL5889159 | — | Ki | = | 150.0 | nM | 6.82 |
| CHEMBL5756552 | — | Ki | = | 160.0 | nM | 6.80 |
| CHEMBL5793743 | — | Ki | = | 170.0 | nM | 6.77 |
| CHEMBL5763340 | — | Ki | = | 180.0 | nM | 6.75 |
| CHEMBL6024077 | — | Ki | = | 190.0 | nM | 6.72 |
| CHEMBL5815820 | — | Ki | = | 190.0 | nM | 6.72 |
| CHEMBL5799508 | — | Ki | = | 190.0 | nM | 6.72 |
| CHEMBL5852351 | — | Ki | = | 200.0 | nM | 6.70 |
| CHEMBL5790843 | — | Ki | = | 210.0 | nM | 6.68 |
| CHEMBL5934087 | — | Ki | = | 220.0 | nM | 6.66 |
| CHEMBL5840977 | — | Ki | = | 240.0 | nM | 6.62 |
| CHEMBL5797861 | — | Ki | = | 250.0 | nM | 6.60 |
| CHEMBL6000875 | — | Ki | = | 290.0 | nM | 6.54 |
| CHEMBL5935401 | — | Ki | = | 340.0 | nM | 6.47 |
| CHEMBL6064889 | — | Ki | = | 440.0 | nM | 6.36 |
| CHEMBL5882320 | — | Ki | = | 450.0 | nM | 6.35 |