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Assay Detail

CHEMBL615123

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxic Activity was evaluated against 143B (TK-) tumor cells
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type 143B
Confidence 1 — Organism
Curated By Intermediate

Target

143B (CHEMBL614508)
Type CELL-LINE
Organism Homo sapiens

Publication

High-affinity inhibitors of dihydrofolate reductase: antimicrobial and anticancer activities of 7,8-dialkyl-1,3-diaminopyrrolo[3,2-f]quinazolines with small molecular size.
Kuyper LF, Baccanari DP, Jones ML, Hunter RN, Tansik RL, Joyner SS, Boytos CM, Rudolph SK, Knick V, Wilson HR, Caddell JM, Friedman HS, Comley JC, Stables JN.
J Med Chem (1996) 39 : 892 -903
PubMed 8632413 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 8.58 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL170758 1 9.10
CHEMBL34259 METHOTREXATE 4.0 1 8.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL170758 IC50 = 0.8 nM 9.10
CHEMBL34259 METHOTREXATE IC50 = 8.8 nM 8.06