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Assay Detail

CHEMBL616455

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity for human 5-hydroxytryptamine 1B receptor
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Expert

Target

5-hydroxytryptamine receptor 1B (CHEMBL1898)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The selective 5-HT1B receptor inverse agonist 1'-methyl-5-[[2'-methyl-4'-(5-methyl-1,2, 4-oxadiazol-3-yl)biphenyl-4-yl]carbonyl]-2,3,6,7-tetrahydro- spiro[furo[2,3-f]indole-3,4'-piperidine] (SB-224289) potently blocks terminal 5-HT autoreceptor function both in vitro and in vivo.
Gaster LM, Blaney FE, Davies S, Duckworth DM, Ham P, Jenkins S, Jennings AJ, Joiner GF, King FD, Mulholland KR, Wyman PA, Hagan JJ, Hatcher J, Jones BJ, Middlemiss DN, Price GW, Riley G, Roberts C, Routledge C, Selkirk J, Slade PD.
J Med Chem (1998) 41 : 1218 -1235
PubMed 9548813 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 8.38 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL282229 1 9.30
CHEMBL20963 1 9.00
CHEMBL15928 GR-127935 1 9.00
CHEMBL21724 1 8.80
CHEMBL282693 1 8.60
CHEMBL20791 1 8.60
CHEMBL21790 1 8.50
CHEMBL281350 SB-224289 1 8.20
CHEMBL277467 1 8.20
CHEMBL20771 1 7.90
CHEMBL15933 1 7.50
CHEMBL21369 1 6.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL282229 Ki = 0.5012 nM 9.30
CHEMBL20963 Ki = 1.0 nM 9.00
CHEMBL15928 GR-127935 Ki = 1.0 nM 9.00
CHEMBL21724 Ki = 1.585 nM 8.80
CHEMBL282693 Ki = 2.512 nM 8.60
CHEMBL20791 Ki = 2.512 nM 8.60
CHEMBL21790 Ki = 3.162 nM 8.50
CHEMBL281350 SB-224289 Ki = 6.31 nM 8.20
CHEMBL277467 Ki = 6.31 nM 8.20
CHEMBL20771 Ki = 12.59 nM 7.90
CHEMBL15933 Ki = 31.62 nM 7.50
CHEMBL21369 Ki = 125.89 nM 6.90