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Assay Detail

CHEMBL644951

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity against human adenosine A3 receptor expressed in CHO cells by [125I]AB-MECA displacement.
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Adenosine receptor A3 (CHEMBL256)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,2,4-triazolo[4,3-a]quinoxalin-1-one moiety as an attractive scaffold to develop new potent and selective human A3 adenosine receptor antagonists: synthesis, pharmacological, and ligand-receptor modeling studies.
Colotta V, Catarzi D, Varano F, Calabri FR, Lenzi O, Filacchioni G, Martini C, Trincavelli L, Deflorian F, Moro S.
J Med Chem (2004) 47 : 3580 -3590
PubMed 15214785 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 7.05 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL324095 1 9.22
CHEMBL333799 1 8.33
CHEMBL421420 1 8.32
CHEMBL121793 1 7.95
CHEMBL16737 1 7.80
CHEMBL116827 1 7.66
CHEMBL16416 1 7.35
CHEMBL120714 1 7.33
CHEMBL332969 1 7.33
CHEMBL121593 1 7.13
CHEMBL333016 1 7.08
CHEMBL120602 1 6.76
CHEMBL331946 1 6.75
CHEMBL120662 1 6.69
CHEMBL118164 1 6.47
CHEMBL333943 1 6.37
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] 1 5.89
CHEMBL118679 1 5.44
CHEMBL1355736 THEOPHYLLINE 4.0 1 4.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL324095 Ki = 0.6 nM 9.22
CHEMBL333799 Ki = 4.7 nM 8.33
CHEMBL421420 Ki = 4.75 nM 8.32
CHEMBL121793 Ki = 11.2 nM 7.95
CHEMBL16737 Ki = 16.0 nM 7.80
CHEMBL116827 Ki = 22.0 nM 7.66
CHEMBL16416 Ki = 45.0 nM 7.35
CHEMBL120714 Ki = 47.0 nM 7.33
CHEMBL332969 Ki = 47.0 nM 7.33
CHEMBL121593 Ki = 73.2 nM 7.13
CHEMBL333016 Ki = 83.0 nM 7.08
CHEMBL120602 Ki = 175.0 nM 6.76
CHEMBL331946 Ki = 176.0 nM 6.75
CHEMBL120662 Ki = 203.0 nM 6.69
CHEMBL118164 Ki = 335.0 nM 6.47
CHEMBL333943 Ki = 429.0 nM 6.37
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] Ki = 1300.0 nM 5.89
CHEMBL118679 Ki = 3600.0 nM 5.44
CHEMBL1355736 THEOPHYLLINE Ki = 86000.0 nM 4.07