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Assay Detail

CHEMBL646055

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Concentration required for the in vitro inhibition of Acyl coenzyme A:cholesterol acyltransferase (ACAT) in liver microsomes isolated from cholesterol-fed rats was determined
9
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Sterol O-acyltransferase 1 (CHEMBL285)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Inhibitors of acyl-CoA: Cholesterol O-acyl transferase (ACAT) as hypocholesterolemic agents. 13. Design, synthesis and biological evaluation of tetrazole anilides as potent inhibitors of ACAT in vitro and hypocholesterolemic agents in vivo
O'Brien PM, Sliskovic DR, Bernabei A, Hurley T, Anderson MK, Bousley RF, Krause BR, Stanfield RL
Bioorg Med Chem Lett (1995) 5 : 295 -300
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.31 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL306344 1 8.00
CHEMBL77370 3 7.85
CHEMBL102912 1 7.66
CHEMBL100448 1 7.28
CHEMBL103748 1 7.10
CHEMBL22373 CL-976 1 6.96
CHEMBL318600 1 6.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL306344 IC50 = 10.0 nM 8.00
CHEMBL77370 IC50 = 14.0 nM 7.85
CHEMBL102912 IC50 = 22.0 nM 7.66
CHEMBL77370 IC50 = 24.0 nM 7.62
CHEMBL100448 IC50 = 52.0 nM 7.28
CHEMBL103748 IC50 = 79.0 nM 7.10
CHEMBL22373 CL-976 IC50 = 110.0 nM 6.96
CHEMBL318600 IC50 = 160.0 nM 6.80
CHEMBL77370 IC50 = 330.0 nM 6.48