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Assay Detail

CHEMBL646568

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [125I]MAdCAM-Ig binding to human integrin alpha4-beta7 of RPMI-8866 cells
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type RPMI-8866
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Integrin alpha-4/beta-7 (CHEMBL2095184)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

N-aryl-prolyl-dipeptides as potent antagonists of VLA-4.
Kamenecka TM, Lanza T, de Laszlo SE, Li B, McCauley ED, Van Riper G, Egger LA, Kidambi U, Mumford RA, Tong S, MacCoss M, Schmidt JA, Hagmann WK.
Bioorg Med Chem Lett (2002) 12 : 2205 -2208
PubMed 12127538 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.92 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL69035 1 8.60
CHEMBL307096 1 8.46
CHEMBL305408 1 8.30
CHEMBL69352 1 8.26
CHEMBL308458 1 8.24
CHEMBL70107 1 8.05
CHEMBL71554 1 7.77
CHEMBL367599 1 7.77
CHEMBL422054 1 7.75
CHEMBL68592 1 7.73
CHEMBL69888 1 7.68
CHEMBL71705 1 7.35
CHEMBL71577 1 6.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL69035 IC50 = 2.5 nM 8.60
CHEMBL307096 IC50 = 3.5 nM 8.46
CHEMBL305408 IC50 = 5.0 nM 8.30
CHEMBL69352 IC50 = 5.5 nM 8.26
CHEMBL308458 IC50 = 5.8 nM 8.24
CHEMBL70107 IC50 = 8.9 nM 8.05
CHEMBL71554 IC50 = 17.1 nM 7.77
CHEMBL367599 IC50 = 17.0 nM 7.77
CHEMBL422054 IC50 = 18.0 nM 7.75
CHEMBL68592 IC50 = 18.8 nM 7.73
CHEMBL69888 IC50 = 21.0 nM 7.68
CHEMBL71705 IC50 = 45.0 nM 7.35
CHEMBL71577 IC50 = 110.0 nM 6.96