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Assay Detail

CHEMBL646639

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]AB-MECA from adenosine A3 receptor from HEK293 cell membranes
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Adenosine receptor A3 (CHEMBL256)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationships and molecular modeling of 3, 5-diacyl-2,4-dialkylpyridine derivatives as selective A3 adenosine receptor antagonists.
Li AH, Moro S, Melman N, Ji XD, Jacobson KA.
J Med Chem (1998) 41 : 3186 -3201
PubMed 9703464 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 7.12 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL431733 NAMODENOSON 3.0 1 9.48
CHEMBL119709 IB-MECA 1 8.96
CHEMBL133566 1 8.82
CHEMBL265096 1 8.57
CHEMBL2112485 1 7.65
CHEMBL2112486 1 7.56
CHEMBL2112484 1 7.30
CHEMBL2112483 1 6.64
CHEMBL107155 1 5.68
CHEMBL105764 1 5.64
CHEMBL107987 1 5.55
CHEMBL108753 1 5.55
CHEMBL100071 1 5.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL431733 NAMODENOSON Ki = 0.33 nM 9.48
CHEMBL119709 IB-MECA Ki = 1.1 nM 8.96
CHEMBL133566 Ki = 1.5 nM 8.82
CHEMBL265096 Ki = 2.69 nM 8.57
CHEMBL2112485 Ki = 22.5 nM 7.65
CHEMBL2112486 Ki = 27.7 nM 7.56
CHEMBL2112484 Ki = 50.5 nM 7.30
CHEMBL2112483 Ki = 229.0 nM 6.64
CHEMBL107155 Ki = 2110.0 nM 5.68
CHEMBL105764 Ki = 2270.0 nM 5.64
CHEMBL107987 Ki = 2830.0 nM 5.55
CHEMBL108753 Ki = 2800.0 nM 5.55
CHEMBL100071 Ki = 7240.0 nM 5.14