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Assay Detail

CHEMBL650829

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity towards Benzodiazepine receptor from bovine brain membrane using [3H]Ro-151788 as radioligand
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL2093872)
Type PROTEIN COMPLEX GROUP
Organism Homo sapiens

Publication

Benzodiazepine receptor ligands. 7. Synthesis and pharmacological evaluation of new 3-esters of the 8-chloropyrazolo[5,1-c][1,2,4]benzotriazine 5-oxide. 3-(2-Thienylmethoxycarbonyl) derivative: an anxioselective agent in rodents.
Costanzo A, Guerrini G, Ciciani G, Bruni F, Costagli C, Selleri S, Besnard F, Costa B, Martini C, Malmberg-Aiello P.
J Med Chem (2002) 45 : 5710 -5720
PubMed 12477354 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 7.64 9.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL407 FLUMAZENIL 4.0 1 9.05
CHEMBL348367 1 9.00
CHEMBL154342 1 8.77
CHEMBL346677 1 8.20
CHEMBL421983 1 8.17
CHEMBL12 DIAZEPAM 4.0 1 8.00
CHEMBL154754 1 7.94
CHEMBL153980 1 7.88
CHEMBL157109 1 7.61
CHEMBL422158 1 7.36
CHEMBL153979 1 6.86
CHEMBL156846 1 6.77
CHEMBL153195 1 6.57
CHEMBL153252 1 6.31
CHEMBL346379 1 6.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL407 FLUMAZENIL Ki = 0.9 nM 9.05
CHEMBL348367 Ki = 1.0 nM 9.00
CHEMBL154342 Ki = 1.7 nM 8.77
CHEMBL346677 Ki = 6.26 nM 8.20
CHEMBL421983 Ki = 6.8 nM 8.17
CHEMBL12 DIAZEPAM Ki = 10.0 nM 8.00
CHEMBL154754 Ki = 11.4 nM 7.94
CHEMBL153980 Ki = 13.3 nM 7.88
CHEMBL157109 Ki = 24.4 nM 7.61
CHEMBL422158 Ki = 44.0 nM 7.36
CHEMBL153979 Ki = 138.0 nM 6.86
CHEMBL156846 Ki = 171.0 nM 6.77
CHEMBL153195 Ki = 272.0 nM 6.57
CHEMBL153252 Ki = 489.0 nM 6.31
CHEMBL346379 Ki = 680.0 nM 6.17