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Assay Detail

CHEMBL653673

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Calpain 1 by [3H]acetyl-casein assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 6 — Multiple protein complex / RNA
Curated By Expert

Target

Calpain 1 (CHEMBL2111357)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Characterization of a continuous fluorogenic assay for calpain I. Kinetic evaluation of peptide aldehydes, halomethyl ketones and (acyloxy)methyl ketones as inhibitors of the enzyme
Harris AL, Gregory JS, Maycock AL, Graybill TL, Osifo I, Schmidt SJ, Dolle RE
Bioorg Med Chem Lett (1995) 5 : 393 -398
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.10 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL341222 1 7.70
CHEMBL340020 1 7.60
CHEMBL338859 1 7.52
CHEMBL341014 1 7.46
CHEMBL286722 1 7.40
CHEMBL423112 1 7.40
CHEMBL419423 1 7.26
CHEMBL129657 1 7.00
CHEMBL339000 1 7.00
CHEMBL127613 1 7.00
CHEMBL128775 1 7.00
CHEMBL128776 1 6.85
CHEMBL129458 1 6.57
CHEMBL128135 1 6.40
CHEMBL129823 1 6.30
CHEMBL127663 1 -
CHEMBL2370670 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL341222 IC50 = 20.0 nM 7.70
CHEMBL340020 IC50 = 25.0 nM 7.60
CHEMBL338859 IC50 = 30.0 nM 7.52
CHEMBL341014 IC50 = 35.0 nM 7.46
CHEMBL286722 IC50 = 40.0 nM 7.40
CHEMBL423112 IC50 = 40.0 nM 7.40
CHEMBL419423 IC50 = 55.0 nM 7.26
CHEMBL129657 IC50 = 100.0 nM 7.00
CHEMBL339000 IC50 = 100.0 nM 7.00
CHEMBL127613 IC50 = 100.0 nM 7.00
CHEMBL128775 IC50 = 100.0 nM 7.00
CHEMBL128776 IC50 = 140.0 nM 6.85
CHEMBL129458 IC50 = 270.0 nM 6.57
CHEMBL128135 IC50 = 400.0 nM 6.40
CHEMBL129823 IC50 = 500.0 nM 6.30
CHEMBL127663 IC50 > 1000.0 nM -
CHEMBL2370670 IC50 > 1000.0 nM -