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Assay Detail

CHEMBL653772

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro vasorelaxant activity (calcium channel blocking activity) was determined with potassium-depolarized rabbit thoracic aorta
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Tissue Thoracic aorta
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Voltage-gated L-type calcium channel (CHEMBL2095229)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Dihydropyrimidine calcium channel blockers. 2. 3-substituted-4-aryl-1,4-dihydro-6-methyl-5-pyrimidinecarboxylic acid esters as potent mimics of dihydropyridines.
Atwal KS, Rovnyak GC, Kimball SD, Floyd DM, Moreland S, Swanson BN, Gougoutas JZ, Schwartz J, Smillie KM, Malley MF.
J Med Chem (1990) 33 : 2629 -2635
PubMed 2391701 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.59 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL475534 NITRENDIPINE 3.0 1 9.00
CHEMBL2092901 1 8.70
CHEMBL3392282 1 8.40
CHEMBL440695 1 7.77
CHEMBL3392280 1 7.58
CHEMBL87921 1 7.58
CHEMBL261982 1 7.30
CHEMBL39925 1 6.89
CHEMBL88449 1 6.57
CHEMBL88149 1 6.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL475534 NITRENDIPINE IC50 = 1.0 nM 9.00
CHEMBL2092901 IC50 = 2.0 nM 8.70
CHEMBL3392282 IC50 = 4.0 nM 8.40
CHEMBL440695 IC50 = 17.0 nM 7.77
CHEMBL3392280 IC50 = 26.0 nM 7.58
CHEMBL87921 IC50 = 26.0 nM 7.58
CHEMBL261982 IC50 = 50.0 nM 7.30
CHEMBL39925 IC50 = 130.0 nM 6.89
CHEMBL88449 IC50 = 270.0 nM 6.57
CHEMBL88149 IC50 = 710.0 nM 6.15