Assay Detail

CHEMBL654086

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding

Inhibitory activity tested against human calpain 1 in Molt-4 cells (intact human T-cell leukemia cell line)

ChEMBL ↗

Free Research Context This assay will appear in recent viewed items on this browser. Open the demo workspace to preview project-only review queues.

Research home Demo workspace

Target

Calpain-1 catalytic subunit (CHEMBL3891) ↗

Open DB target context

Use one target anchor to keep assay intent, target linkage, and tested compound review aligned.

Loading context

Calpain-1 catalytic subunit (CHEMBL3891) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target Calpain-1 catalytic subunit

CHEMBL3891

Anchored from Assay target.

Protein identity Calpain-1 catalytic subunit

Disease frame Disease evidence pending

Open the live target snapshot or evidence card before reviewing assay metadata so target fit and disease framing stay reusable.

Review focus Open DB context pending

Open the live target snapshot before relying on this target frame across surface handoff.

Open target snapshot Open evidence card Search with this context

Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Binding
Cell Type	MOLT-4
Confidence	8 — Homologous single protein target
Curated By	Autocuration

Target

Calpain-1 catalytic subunit (CHEMBL3891) ↗

Type	SINGLE PROTEIN
Organism	Homo sapiens

Publication

1,2-Benzothiazine 1,1-dioxide alpha-ketoamide analogues as potent calpain I inhibitors.

Bihovsky R, Tao M, Mallamo JP, Wells GJ.
Bioorg Med Chem Lett (2004) 14 : 1035 -1038
PubMed 15013018 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	1	8.68	8.68

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL8651	—	—	1	8.68

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL8651	—	IC50	=	2.1	nM	8.68