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Assay Detail

CHEMBL658554

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours)
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cricetulus griseus
Cell Type CHO-AA8
Confidence 1 — Organism
Curated By Intermediate

Target

CHO-AA8 (CHEMBL614555)
Type CELL-LINE
Organism Cricetulus griseus

Publication

Hypoxia-selective antitumor agents. 2. Electronic effects of 4-substituents on the mechanisms of cytotoxicity and metabolic stability of nitracrine derivatives.
Wilson WR, Thompson LH, Anderson RF, Denny WA.
J Med Chem (1989) 32 : 31 -38
PubMed 2909741 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 8.11 9.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL127655 4-NITROQUINOLINE-1-OXIDE 1 9.48
CHEMBL43 AMSACRINE 4.0 1 9.22
CHEMBL105 MITOMYCIN 4.0 1 8.97
CHEMBL124006 1 8.37
CHEMBL515 CHLORAMBUCIL 4.0 1 7.44
CHEMBL338686 METHANESULFONIC ACID ETHYL ESTER 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL127655 4-NITROQUINOLINE-1-OXIDE IC50 = 0.33 nM 9.48
CHEMBL43 AMSACRINE IC50 = 0.6 nM 9.22
CHEMBL105 MITOMYCIN IC50 = 1.08 nM 8.97
CHEMBL124006 IC50 = 4.3 nM 8.37
CHEMBL515 CHLORAMBUCIL IC50 = 36.0 nM 7.44
CHEMBL338686 METHANESULFONIC ACID ETHYL ESTER IC50 = 6800.0 nM 5.17