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Assay Detail

CHEMBL662240

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
The inhibitory concentration of compound against Dihydrofolate reductases on Toxoplasma gondii
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Toxoplasma gondii
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Bifunctional dihydrofolate reductase-thymidylate synthase (CHEMBL2425)
Type SINGLE PROTEIN
Organism Toxoplasma gondii

Publication

2-amino-4-oxo-5-substituted-pyrrolo[2,3-d]pyrimidines as nonclassical antifolate inhibitors of thymidylate synthase.
Gangjee A, Mavandadi F, Kisliuk RL, McGuire JJ, Queener SF.
J Med Chem (1996) 39 : 4563 -4568
PubMed 8917644 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.05 5.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL142176 1 5.51
CHEMBL400485 1 4.93
CHEMBL140323 1 4.70
CHEMBL400364 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL142176 IC50 = 3100.0 nM 5.51
CHEMBL400485 IC50 = 11700.0 nM 4.93
CHEMBL140323 IC50 = 20000.0 nM 4.70
CHEMBL400364 IC50 = 244000.0 nM -