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Assay Detail

CHEMBL663135

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Concentration required for 50% inhibition against dihydrofolate reductase of Streptococcus faecium
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Enterococcus faecium
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Folate analogues. 21. Synthesis and antifolate and antitumor activities of N10-(cyanomethyl)-5,8-dideazafolic acid.
Nair MG, Salter DC, Kisliuk RL, Gaumont Y, North G, Sirotnak FM.
J Med Chem (1983) 26 : 605 -607
PubMed 6403710 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.26 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 8.92
CHEMBL422395 1 6.52
CHEMBL34918 1 6.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 1.2 nM 8.92
CHEMBL422395 IC50 = 300.0 nM 6.52
CHEMBL34918 IC50 = 460.0 nM 6.34