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Assay Detail

CHEMBL663689

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against dihydrofolate reductase (DHFR) enzyme from chicken liver
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Gallus gallus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL2575)
Type SINGLE PROTEIN
Organism Gallus gallus

Publication

2,4-Diamino-5-benzylpyrimidines as antibacterial agents. 8. The 3,4,5-triethyl isostere of trimethoprim. A study of specificity.
Roth B, Aig E.
J Med Chem (1987) 30 : 1998 -2004
PubMed 3312605 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.48 4.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL57582 1 4.48
CHEMBL19633 DIAVERIDINE 2.0 1 -
CHEMBL293013 1 -
CHEMBL22 TRIMETHOPRIM 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL57582 IC50 = 33000.0 nM 4.48
CHEMBL19633 DIAVERIDINE IC50 = 300000.0 nM -
CHEMBL293013 IC50 = 120000.0 nM -
CHEMBL22 TRIMETHOPRIM IC50 = 730000.0 nM -