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Assay Detail

CHEMBL664186

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against DHFR (Dihydrofolate reductase) from Pneumocystis carinii.
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pneumocystis carinii
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL1926)
Type SINGLE PROTEIN
Organism Pneumocystis carinii

Publication

Structure-based design of selective inhibitors of dihydrofolate reductase: synthesis and antiparasitic activity of 2, 4-diaminopteridine analogues with a bridged diarylamine side chain.
Rosowsky A, Cody V, Galitsky N, Fu H, Papoulis AT, Queener SF.
J Med Chem (1999) 42 : 4853 -4860
PubMed 10579848 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.32 7.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL144839 1 7.38
CHEMBL119 TRIMETREXATE 4.0 1 7.38
CHEMBL423200 1 7.00
CHEMBL144200 1 6.92
CHEMBL301769 1 6.68
CHEMBL342086 1 5.85
CHEMBL423381 1 5.47
CHEMBL424113 1 5.31
CHEMBL22 TRIMETHOPRIM 4.0 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL144839 IC50 = 42.0 nM 7.38
CHEMBL119 TRIMETREXATE IC50 = 42.0 nM 7.38
CHEMBL423200 IC50 = 100.0 nM 7.00
CHEMBL144200 IC50 = 120.0 nM 6.92
CHEMBL301769 IC50 = 210.0 nM 6.68
CHEMBL342086 IC50 = 1400.0 nM 5.85
CHEMBL423381 IC50 = 3400.0 nM 5.47
CHEMBL424113 IC50 = 4900.0 nM 5.31
CHEMBL22 TRIMETHOPRIM IC50 = 12000.0 nM 4.92