Assay Detail
Binding
CHEMBL664361
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Evaluated for the inhibition of chicken liver Dihydrofolate reductase by using standard enzyme assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Gallus gallus |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Publication
Lysine and ornithine analogues of methotrexate as inhibitors of dihydrofolate reductase.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 6.42 | 7.05 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.05 |
| CHEMBL418165 | — | — | 1 | 6.89 |
| CHEMBL36083 | — | — | 1 | 6.60 |
| CHEMBL356340 | — | — | 1 | 6.48 |
| CHEMBL142626 | — | — | 1 | 6.42 |
| CHEMBL342744 | — | — | 1 | 6.02 |
| CHEMBL146440 | — | — | 1 | 5.51 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL418165 | — | IC50 | = | 130.0 | nM | 6.89 |
| CHEMBL36083 | — | IC50 | = | 250.0 | nM | 6.60 |
| CHEMBL356340 | — | IC50 | = | 330.0 | nM | 6.48 |
| CHEMBL142626 | — | IC50 | = | 380.0 | nM | 6.42 |
| CHEMBL342744 | — | IC50 | = | 950.0 | nM | 6.02 |
| CHEMBL146440 | — | IC50 | = | 3100.0 | nM | 5.51 |