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Assay Detail

CHEMBL664374

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Binding
Inhibition against Dihydrofolate reductase in Pneumocystis carinii
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pneumocystis carinii
Confidence 9 — Direct single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL1926)
Type SINGLE PROTEIN
Organism Pneumocystis carinii

Publication

Effect of N9-methylation and bridge atom variation on the activity of 5-substituted 2,4-diaminopyrrolo[2,3-d]pyrimidines against dihydrofolate reductases from Pneumocystis carinii and Toxoplasma gondii.
Gangjee A, Mavandadi F, Queener SF.
J Med Chem (1997) 40 : 1173 -1177
PubMed 9089339 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.92 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 9.00
CHEMBL17232 1 7.42
CHEMBL7492 PIRITREXIM 2.0 1 7.42
CHEMBL119 TRIMETREXATE 4.0 1 7.38
CHEMBL17287 1 7.36
CHEMBL17479 1 4.97
CHEMBL17431 1 4.96
CHEMBL22 TRIMETHOPRIM 4.0 1 4.92
CHEMBL278013 1 4.55
CHEMBL17873 1 4.45
CHEMBL17323 1 4.34
CHEMBL17430 1 4.23
CHEMBL17453 1 -
CHEMBL276734 1 -
CHEMBL275893 1 -
CHEMBL278261 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 1.0 nM 9.00
CHEMBL17232 IC50 = 38.0 nM 7.42
CHEMBL7492 PIRITREXIM IC50 = 38.0 nM 7.42
CHEMBL119 TRIMETREXATE IC50 = 42.0 nM 7.38
CHEMBL17287 IC50 = 44.0 nM 7.36
CHEMBL17479 IC50 = 10600.0 nM 4.97
CHEMBL17431 IC50 = 11100.0 nM 4.96
CHEMBL22 TRIMETHOPRIM IC50 = 12000.0 nM 4.92
CHEMBL278013 IC50 = 28300.0 nM 4.55
CHEMBL17873 IC50 = 35300.0 nM 4.45
CHEMBL17323 IC50 = 45700.0 nM 4.34
CHEMBL17430 IC50 = 58500.0 nM 4.23
CHEMBL17453 IC50 > 12000.0 nM -
CHEMBL276734 IC50 = 307000.0 nM -
CHEMBL275893 IC50 = 929000.0 nM -
CHEMBL278261 IC50 = 209000.0 nM -