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Assay Detail

CHEMBL664376

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Dihydrofolate reductase of Pneumocystis carinii
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pneumocystis carinii
Confidence 9 — Direct single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL1926)
Type SINGLE PROTEIN
Organism Pneumocystis carinii

Publication

2,4-Diamino-5-chloroquinazoline analogues of trimetrexate and piritrexim: synthesis and antifolate activity.
Rosowsky A, Mota CE, Wright JE, Queener SF.
J Med Chem (1994) 37 : 4522 -4528
PubMed 7799402 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.24 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL328897 1 7.92
CHEMBL7492 PIRITREXIM 2.0 1 7.51
CHEMBL88555 1 7.48
CHEMBL137942 1 7.48
CHEMBL119 TRIMETREXATE 4.0 1 7.38
CHEMBL88430 1 7.29
CHEMBL138060 1 7.28
CHEMBL138265 1 6.77
CHEMBL36 PYRIMETHAMINE 4.0 1 5.43
CHEMBL22 TRIMETHOPRIM 4.0 1 4.92
CHEMBL141903 1 4.92
CHEMBL138625 1 4.58
CHEMBL141417 1 4.37
CHEMBL141469 1 4.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL328897 IC50 = 12.0 nM 7.92
CHEMBL7492 PIRITREXIM IC50 = 31.0 nM 7.51
CHEMBL88555 IC50 = 33.0 nM 7.48
CHEMBL137942 IC50 = 33.0 nM 7.48
CHEMBL119 TRIMETREXATE IC50 = 42.0 nM 7.38
CHEMBL88430 IC50 = 51.0 nM 7.29
CHEMBL138060 IC50 = 53.0 nM 7.28
CHEMBL138265 IC50 = 170.0 nM 6.77
CHEMBL36 PYRIMETHAMINE IC50 = 3700.0 nM 5.43
CHEMBL22 TRIMETHOPRIM IC50 = 12000.0 nM 4.92
CHEMBL141903 IC50 = 12000.0 nM 4.92
CHEMBL138625 IC50 = 26000.0 nM 4.58
CHEMBL141417 IC50 = 43000.0 nM 4.37
CHEMBL141469 IC50 = 95000.0 nM 4.02