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Assay Detail

CHEMBL665036

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibition of Pneumocystis carinii (Pc) dihydrofolate reductase.
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL2364669)
Type SINGLE PROTEIN
Organism Bacteria

Publication

Inhibition of Pneumocystis carinii, Toxoplasma gondii, and Mycobacterium avium dihydrofolate reductases by 2,4-diamino-5-[2-methoxy-5-(omega-carboxyalkyloxy)benzyl]pyrimidines: marked improvement in potency relative to trimethoprim and species selectivity relative to piritrexim.
Rosowsky A, Forsch RA, Queener SF.
J Med Chem (2002) 45 : 233 -241
PubMed 11754594 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 5.56 7.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL7492 PIRITREXIM 2.0 1 7.51
CHEMBL37520 1 7.31
CHEMBL55764 1 6.77
CHEMBL37696 1 6.60
CHEMBL283515 1 6.19
CHEMBL285448 1 6.10
CHEMBL48203 1 5.77
CHEMBL36310 1 5.58
CHEMBL301769 1 5.58
CHEMBL280378 EPIROPRIM 2.0 1 5.58
CHEMBL38332 1 5.32
CHEMBL287481 1 5.25
CHEMBL289904 1 5.15
CHEMBL23960 1 5.11
CHEMBL22434 1 5.02
CHEMBL22 TRIMETHOPRIM 4.0 1 4.92
CHEMBL284851 1 4.85
CHEMBL79890 1 4.72
CHEMBL312688 1 4.70
CHEMBL78648 1 4.36
CHEMBL420384 1 4.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL7492 PIRITREXIM IC50 = 31.0 nM 7.51
CHEMBL37520 IC50 = 49.0 nM 7.31
CHEMBL55764 IC50 = 170.0 nM 6.77
CHEMBL37696 IC50 = 250.0 nM 6.60
CHEMBL283515 IC50 = 650.0 nM 6.19
CHEMBL285448 IC50 = 800.0 nM 6.10
CHEMBL48203 IC50 = 1700.0 nM 5.77
CHEMBL36310 IC50 = 2600.0 nM 5.58
CHEMBL301769 IC50 = 2600.0 nM 5.58
CHEMBL280378 EPIROPRIM IC50 = 2600.0 nM 5.58
CHEMBL38332 IC50 = 4800.0 nM 5.32
CHEMBL287481 IC50 = 5600.0 nM 5.25
CHEMBL289904 IC50 = 7100.0 nM 5.15
CHEMBL23960 IC50 = 7700.0 nM 5.11
CHEMBL22434 IC50 = 9500.0 nM 5.02
CHEMBL22 TRIMETHOPRIM IC50 = 12000.0 nM 4.92
CHEMBL284851 IC50 = 14000.0 nM 4.85
CHEMBL79890 IC50 = 19000.0 nM 4.72
CHEMBL312688 IC50 = 20000.0 nM 4.70
CHEMBL78648 IC50 = 44000.0 nM 4.36
CHEMBL420384 IC50 = 51000.0 nM 4.29