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Assay Detail

CHEMBL665040

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Pneumocystis carinii dihydrofolate reductase
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL1926)
Type SINGLE PROTEIN
Organism Pneumocystis carinii

Publication

Synthesis of 5-methyl-5-deaza nonclassical antifolates as inhibitors of dihydrofolate reductases and as potential antipneumocystis, antitoxoplasma, and antitumor agents.
Gangjee A, Shi J, Queener SF, Barrows LR, Kisliuk RL.
J Med Chem (1993) 36 : 3437 -3443
PubMed 8230134 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.93 7.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL50514 1 7.88
CHEMBL7492 PIRITREXIM 2.0 1 7.42
CHEMBL119 TRIMETREXATE 4.0 1 7.38
CHEMBL420224 1 7.20
CHEMBL23532 1 7.07
CHEMBL82975 1 7.00
CHEMBL82924 1 6.98
CHEMBL81820 1 6.50
CHEMBL116614 1 6.29
CHEMBL334271 1 6.28
CHEMBL115788 1 6.26
CHEMBL421053 1 -
CHEMBL114668 1 -
CHEMBL117172 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL50514 IC50 = 13.2 nM 7.88
CHEMBL7492 PIRITREXIM IC50 = 38.0 nM 7.42
CHEMBL119 TRIMETREXATE IC50 = 42.0 nM 7.38
CHEMBL420224 IC50 = 63.0 nM 7.20
CHEMBL23532 IC50 = 86.0 nM 7.07
CHEMBL82975 IC50 = 100.0 nM 7.00
CHEMBL82924 IC50 = 104.5 nM 6.98
CHEMBL81820 IC50 = 320.0 nM 6.50
CHEMBL116614 IC50 = 510.0 nM 6.29
CHEMBL334271 IC50 = 520.0 nM 6.28
CHEMBL115788 IC50 = 550.0 nM 6.26
CHEMBL421053 IC50 > 3700.0 nM -
CHEMBL114668 IC50 > 2600.0 nM -
CHEMBL117172 IC50 > 1200.0 nM -