Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL665585

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Dihydrofolate reductase from Toxoplasma gondii (tgDHFR)
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Toxoplasma gondii
Confidence 9 — Direct single protein target
Curated By Expert

Target

Bifunctional dihydrofolate reductase-thymidylate synthase (CHEMBL2425)
Type SINGLE PROTEIN
Organism Toxoplasma gondii

Publication

Structure-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents.
Gangjee A, Vidwans AP, Vasudevan A, Queener SF, Kisliuk RL, Cody V, Li R, Galitsky N, Luft JR, Pangborn W.
J Med Chem (1998) 41 : 3426 -3434
PubMed 9719595 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.91 8.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL325434 1 8.41
CHEMBL115804 1 8.41
CHEMBL114354 1 8.39
CHEMBL113816 1 8.33
CHEMBL117750 1 8.27
CHEMBL114839 1 7.05
CHEMBL114833 1 6.27
CHEMBL114058 1 6.12
CHEMBL116200 1 6.11
CHEMBL116637 1 6.09
CHEMBL116815 1 6.08
CHEMBL113999 1 6.04
CHEMBL116816 1 5.96
CHEMBL119 TRIMETREXATE 4.0 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL325434 IC50 = 3.9 nM 8.41
CHEMBL115804 IC50 = 3.9 nM 8.41
CHEMBL114354 IC50 = 4.1 nM 8.39
CHEMBL113816 IC50 = 4.7 nM 8.33
CHEMBL117750 IC50 = 5.4 nM 8.27
CHEMBL114839 IC50 = 90.0 nM 7.05
CHEMBL114833 IC50 = 540.0 nM 6.27
CHEMBL114058 IC50 = 750.0 nM 6.12
CHEMBL116200 IC50 = 780.0 nM 6.11
CHEMBL116637 IC50 = 810.0 nM 6.09
CHEMBL116815 IC50 = 840.0 nM 6.08
CHEMBL113999 IC50 = 920.0 nM 6.04
CHEMBL116816 IC50 = 1100.0 nM 5.96
CHEMBL119 TRIMETREXATE IC50 = 6800.0 nM 5.17