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Assay Detail

CHEMBL665604

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against purified DHFR (Dihydrofolate reductase) from Toxoplasma gondii.
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Toxoplasma gondii
Confidence 9 — Direct single protein target
Curated By Expert

Target

Bifunctional dihydrofolate reductase-thymidylate synthase (CHEMBL2425)
Type SINGLE PROTEIN
Organism Toxoplasma gondii

Publication

Nonclassical 2,4-diamino-6-(aminomethyl)-5,6,7,8-tetrahydroquinazoline antifolates: synthesis and biological activities.
Gangjee A, Zaveri N, Kothare M, Queener SF.
J Med Chem (1995) 38 : 3660 -3668
PubMed 7658454 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.18 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL333791 1 8.00
CHEMBL120826 1 7.57
CHEMBL7488 1 7.27
CHEMBL121947 1 7.11
CHEMBL121319 1 7.01
CHEMBL120939 1 6.85
CHEMBL122704 1 6.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL333791 IC50 = 9.9 nM 8.00
CHEMBL120826 IC50 = 27.0 nM 7.57
CHEMBL7488 IC50 = 54.0 nM 7.27
CHEMBL121947 IC50 = 78.0 nM 7.11
CHEMBL121319 IC50 = 97.0 nM 7.01
CHEMBL120939 IC50 = 140.0 nM 6.85
CHEMBL122704 IC50 = 330.0 nM 6.48