Assay Detail
Binding
CHEMBL665613
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Inhibitory activity against Toxoplasma gondii dihydrofolate reductase (in 50 uM dihydrofolic acid)
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Toxoplasma gondii |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Bifunctional dihydrofolate reductase-thymidylate synthase (CHEMBL2425) ↗| Type | SINGLE PROTEIN |
| Organism | Toxoplasma gondii |
Publication
2,4-diamino-5-deaza-6-substituted pyrido[2,3-d]pyrimidine antifolates as potent and selective nonclassical inhibitors of dihydrofolate reductases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 7.14 | 7.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.66 |
| CHEMBL35222 | — | — | 1 | 7.58 |
| CHEMBL32113 | — | — | 1 | 7.55 |
| CHEMBL36245 | — | — | 1 | 7.54 |
| CHEMBL33038 | — | — | 1 | 7.46 |
| CHEMBL32708 | — | — | 1 | 6.30 |
| CHEMBL33208 | — | — | 1 | 5.89 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 22.0 | nM | 7.66 |
| CHEMBL35222 | — | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL32113 | — | IC50 | = | 28.0 | nM | 7.55 |
| CHEMBL36245 | — | IC50 | = | 29.0 | nM | 7.54 |
| CHEMBL33038 | — | IC50 | = | 35.0 | nM | 7.46 |
| CHEMBL32708 | — | IC50 | = | 500.0 | nM | 6.30 |
| CHEMBL33208 | — | IC50 | = | 1300.0 | nM | 5.89 |