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Assay Detail

CHEMBL665621

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Dihydrofolate reductase from humans.
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma brucei
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and evaluation of inhibitors of trypanosomal and leishmanial dihydrofolate reductase.
Chowdhury SF, Villamor VB, Guerrero RH, Leal I, Brun R, Croft SL, Goodman JM, Maes L, Ruiz-Perez LM, Pacanowska DG, Gilbert IH.
J Med Chem (1999) 42 : 4300 -4312
PubMed 10543874 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 7.83 8.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL31946 1 8.44
CHEMBL326989 1 8.19
CHEMBL137305 1 8.15
CHEMBL137448 1 8.07
CHEMBL334919 1 8.06
CHEMBL18967 1 8.02
CHEMBL31547 1 8.01
CHEMBL343271 1 8.01
CHEMBL22 TRIMETHOPRIM 4.0 1 8.00
CHEMBL343127 1 7.98
CHEMBL36 PYRIMETHAMINE 4.0 1 7.96
CHEMBL274031 1 7.96
CHEMBL344592 1 7.89
CHEMBL135935 1 7.72
CHEMBL31235 1 7.62
CHEMBL137641 1 7.44
CHEMBL334695 1 7.28
CHEMBL138230 1 7.12
CHEMBL285209 1 6.88

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL31946 Ki = 3.6 nM 8.44
CHEMBL326989 Ki = 6.4 nM 8.19
CHEMBL137305 Ki = 7.1 nM 8.15
CHEMBL137448 Ki = 8.6 nM 8.07
CHEMBL334919 Ki = 8.8 nM 8.06
CHEMBL18967 Ki = 9.6 nM 8.02
CHEMBL31547 Ki = 9.8 nM 8.01
CHEMBL343271 Ki = 9.8 nM 8.01
CHEMBL22 TRIMETHOPRIM Ki = 10.0 nM 8.00
CHEMBL343127 Ki = 10.5 nM 7.98
CHEMBL36 PYRIMETHAMINE Ki = 11.0 nM 7.96
CHEMBL274031 Ki = 11.0 nM 7.96
CHEMBL344592 Ki = 13.0 nM 7.89
CHEMBL135935 Ki = 19.0 nM 7.72
CHEMBL31235 Ki = 24.0 nM 7.62
CHEMBL137641 Ki = 36.0 nM 7.44
CHEMBL334695 Ki = 52.0 nM 7.28
CHEMBL138230 Ki = 75.0 nM 7.12
CHEMBL285209 Ki = 131.0 nM 6.88