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Assay Detail

CHEMBL666806

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Binding
Inhibitory activity against Leu22-Phe mutant human Dihydrofolate reductase
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL202)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2,4-Diamino-5-substituted-quinazolines as inhibitors of a human dihydrofolate reductase with a site-directed mutation at position 22 and of the dihydrofolate reductases from Pneumocystis carinii and Toxoplasma gondii.
Rosowsky A, Mota CE, Queener SF, Waltham M, Ercikan-Abali E, Bertino JR.
J Med Chem (1995) 38 : 745 -752
PubMed 7877140 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 8.09 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL305177 1 11.00
CHEMBL34259 METHOTREXATE 4.0 1 9.96
CHEMBL376180 AMINOPTERIN 2.0 1 9.68
CHEMBL164845 1 8.74
CHEMBL162476 1 8.57
CHEMBL341703 1 8.54
CHEMBL349149 1 8.38
CHEMBL441298 1 8.37
CHEMBL350730 1 7.48
CHEMBL7492 PIRITREXIM 2.0 1 7.42
CHEMBL162270 1 7.31
CHEMBL119 TRIMETREXATE 4.0 1 7.08
CHEMBL355151 1 7.02
CHEMBL162312 1 6.85
CHEMBL434097 1 6.60
CHEMBL165955 1 6.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL305177 Ki = 0.01 nM 11.00
CHEMBL34259 METHOTREXATE Ki = 0.11 nM 9.96
CHEMBL376180 AMINOPTERIN Ki = 0.21 nM 9.68
CHEMBL164845 Ki = 1.8 nM 8.74
CHEMBL162476 Ki = 2.7 nM 8.57
CHEMBL341703 Ki = 2.9 nM 8.54
CHEMBL349149 Ki = 4.2 nM 8.38
CHEMBL441298 Ki = 4.3 nM 8.37
CHEMBL350730 Ki = 33.0 nM 7.48
CHEMBL7492 PIRITREXIM Ki = 38.0 nM 7.42
CHEMBL162270 Ki = 49.0 nM 7.31
CHEMBL119 TRIMETREXATE Ki = 83.0 nM 7.08
CHEMBL355151 Ki = 96.3 nM 7.02
CHEMBL162312 Ki = 140.0 nM 6.85
CHEMBL434097 Ki = 250.0 nM 6.60
CHEMBL165955 Ki = 310.0 nM 6.51